Methods in Molecular Biology

Mammalian Cell Viability

Methods and Protocols

Editors: Stoddart, Martin J. (Ed.)

  • Features protocols utilizing both common lab equipment and specialized, cutting-edge equipment
  • Facilitates the multiplexing of methods for the greatest levels of success
  • Contains expert tips and key implementation advice
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-108-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-61779-107-9
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  • Usually dispatched within 3 to 5 business days.
About this book

Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.  In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.

 

Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Table of contents (21 chapters)

  • Cell Viability Assays: Introduction

    Stoddart, Martin J.

    Pages 1-6

  • Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods

    Louis, Kristine S. (et al.)

    Pages 7-12

  • Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay

    Kupcsik, László

    Pages 13-19

  • WST-8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells

    Stoddart, Martin J.

    Pages 21-25

  • Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye

    Czekanska, Ewa M.

    Pages 27-32

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-108-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-61779-107-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Mammalian Cell Viability
Book Subtitle
Methods and Protocols
Editors
  • Martin J. Stoddart
Series Title
Methods in Molecular Biology
Series Volume
740
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-108-6
DOI
10.1007/978-1-61779-108-6
Hardcover ISBN
978-1-61779-107-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 240
Topics