Methods in Molecular Biology

MicroRNAs in Development

Methods and Protocols

Editors: Dalmay, Tamas (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step details essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-083-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-61779-082-9
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  • Usually dispatched within 3 to 5 business days.
About this book

Since the discovery of microRNAs, developmental biologists have striven to understand the role of miRNAs in development and disease. MicroRNAs in Development: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activity of miRNAs in various organisms, and it concludes with a section that outlines different methods to identify and validate miRNA targets in animals and plants. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, MicroRNAs in Development: Methods and Protocols serves as a practical guide for scientists of all backgrounds and conveys the appropriate sense of fascination associated with this vital field of research.

Reviews

From the reviews:

“Well edited book by Prof. Tamas Dalmay provides a complete (as for today !) overview of the role played by microRNAs … . Thanks to this book I think that a multitude of readers can better understand how the genome express itself in time and space. … gives to the scientific community a clear vision of a five dimensional world in which to investigate: the three anatomical spaces (x, y and z) plus the heterochronic and heterotopic dimensions of gene expressions thanks to the microRNAs.” (Manuela Monti, European Journal of Histochemistry, July, 2011)

Table of contents (15 chapters)

  • In Situ Detection of microRNAs in Animals

    Sweetman, Dylan

    Pages 1-8

  • Detection of microRNAs in Plants by In Situ Hybridisation

    Várallyay, Éva (et al.)

    Pages 9-23

  • Detecting sRNAs by Northern Blotting

    López-Gomollón, Sara

    Pages 25-38

  • Profiling MicroRNAs by Real-Time PCR

    Jacobsen, Nana (et al.)

    Pages 39-54

  • Detection of Small RNAs and MicroRNAs Using Deep Sequencing Technology

    Havecker, Ericka R.

    Pages 55-68

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-083-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-61779-082-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
MicroRNAs in Development
Book Subtitle
Methods and Protocols
Editors
  • Tamas Dalmay
Series Title
Methods in Molecular Biology
Series Volume
732
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-083-6
DOI
10.1007/978-1-61779-083-6
Hardcover ISBN
978-1-61779-082-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 200
Topics