Methods in Molecular Biology

Cancer Cell Culture

Methods and Protocols

Editors: Cree, Ian A. (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides the step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-080-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-61779-079-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve.  Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field.

Table of contents (38 chapters)

  • Cancer Biology

    Cree, Ian A.

    Pages 1-11

  • Principles of Cancer Cell Culture

    Cree, Ian A.

    Pages 13-26

  • Storage of Cell Lines

    Parker, Katharine A.

    Pages 27-34

  • Characterization and Authentication of Cancer Cell Lines: An Overview

    Reid, Yvonne A.

    Pages 35-43

  • Online Verification of Human Cell Line Identity by STR DNA Typing

    Dirks, Wilhelm G. (et al.)

    Pages 45-55

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-080-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-61779-079-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Cancer Cell Culture
Book Subtitle
Methods and Protocols
Editors
  • Ian A. Cree
Series Title
Methods in Molecular Biology
Series Volume
731
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-080-5
DOI
10.1007/978-1-61779-080-5
Hardcover ISBN
978-1-61779-079-9
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIV, 502
Topics