Methods in Molecular Biology

Serum/Plasma Proteomics

Methods and Protocols

Editors: Simpson, Richard J., Greening, David W. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from experts
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-068-3
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  • Immediate eBook download after purchase
Hardcover $169.00
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  • ISBN 978-1-61779-067-6
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About this book

Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring.  Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that allow greater fundamental insights into the blood proteome.  These technological improvements have, in part, fuelled the quest for the discovery of novel blood-based biomarkers of disease. Serum/Plasma Proteomics: Methods and Protocols is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, this detailed volume covers fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays,  proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, and finally data management, statistical design, and bioinformatic challenges. This book, contributed to by leading experts in the field, provides a valuable foundation for the development and application of blood-based proteomics. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Serum/Plasma Proteomics: Methods and Protocols, with its well-honed methodologies, seeks to serve both professionals and investigators new to the field in an effort to further our knowledge of this fundamental science.

Table of contents (23 chapters)

  • Plasma Biomarker Discovery Using 3D Protein Profiling Coupled with Label-Free Quantitation

    Beer, Lynn A. (et al.)

    Pages 3-27

  • Intact Protein Separation by One- and Two-Dimensional Liquid Chromatography for the Comparative Proteomic Separation of Partitioned Serum or Plasma

    Sheng, Simon (et al.)

    Pages 29-46

  • In-Depth Analysis of a Plasma or Serum Proteome Using a 4D Protein Profiling Method

    Tang, Hsin-Yao (et al.)

    Pages 47-67

  • Intact-Protein Analysis System for Discovery of Serum-Based Disease Biomarkers

    Wang, Hong (et al.)

    Pages 69-85

  • Model-Based Discovery of Circulating Biomarkers

    Vogelsang, Maryann S. (et al.)

    Pages 87-107

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-068-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-067-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Serum/Plasma Proteomics
Book Subtitle
Methods and Protocols
Editors
  • Richard J. Simpson
  • David W. Greening
Series Title
Methods in Molecular Biology
Series Volume
728
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-068-3
DOI
10.1007/978-1-61779-068-3
Hardcover ISBN
978-1-61779-067-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 397
Topics