Methods in Molecular Biology

Antiviral RNAi

Concepts, Methods, and Applications

Editors: van Rij, Ronald P. (Ed.)

  • -Includes cutting-edge methods and protocols
  • -Provides step-by-step detail essential for reproducible results
  • -Contains key notes and implementation advice from the experts
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eBook $129.00
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  • ISBN 978-1-61779-036-2
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Softcover $169.00
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  • ISBN 978-1-4939-5825-2
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About this book

Viruses and RNAi share an intricate relationship at many levels. RNAi is an important antiviral defense mechanism in plants and invertebrates, microRNAs – of viral or cellular origin – affect many aspects of virus biology, and replication of many, if not all, mammalian viruses can be suppressed by RNAi. Antiviral RNAi: Concepts, Methods, and Applications provides a collection of protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as for the development and optimization of RNAi-based antiviral drugs. As RNAi is a central regulatory mechanism in the cell, the methods in this volume can also be applied out of the context of a virus infection. Divided into five convenient parts, this detailed volume reviews important basic concepts in the field of antiviral RNAi, provides experimental and bio-informatic tools for the analysis of small silencing RNAs, covers methods to biochemically dissect RNAi-based antiviral defense and viral counter-defense mechanisms, describes methods for the design, expression, and delivery of therapeutic antiviral siRNAs, and finally presents genome-wide RNAi approaches for the identification of factors involved in virus replication. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Antiviral RNAi: Concepts, Methods, and Applications serves as an ideal guide for both novice and experienced researchers alike striving to dissect the role of RNAi in the viral life cycle or to further boost the development of novel therapeutics and experimental tools based on RNAi technology.

Reviews

From the reviews:

“This well-illustrated book details the necessary protocols for the analysis of viral small RNAs and natural antiviral RNAi responses as well as the development and optimization of RNAi-based antiviral drugs. The book is intended for both novice and experienced researchers … . It also will be of immense help to graduate students, physicians who treat patients with infectious diseases, and other researchers interested in the field of antiviral RNAi. … a well-illustrated and authoritative resource on important basic concepts in the field of antiviral RNAi.” (Omer Iqbal, Doody’s Review Service, July, 2011)

Table of contents (26 chapters)

  • Defense and Counterdefense in the RNAi-Based Antiviral Immune System in Insects

    Mierlo, Joël T. (et al.)

    Pages 3-22

  • RNAi and Cellular miRNAs in Infections by Mammalian Viruses

    Haasnoot, Joost (et al.)

    Pages 23-41

  • Viral miRNAs

    Plaisance-Bonstaff, Karlie (et al.)

    Pages 43-66

  • Progress in RNAi-Based Antiviral Therapeutics

    Zhou, Jiehua (et al.)

    Pages 67-75

  • Chemical Modification of Small Interfering RNA

    Bramsen, Jesper B. (et al.)

    Pages 77-103

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-037-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-61779-036-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-5825-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Antiviral RNAi
Book Subtitle
Concepts, Methods, and Applications
Editors
  • Ronald P. van Rij
Series Title
Methods in Molecular Biology
Series Volume
721
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-037-9
DOI
10.1007/978-1-61779-037-9
Hardcover ISBN
978-1-61779-036-2
Softcover ISBN
978-1-4939-5825-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 425
Number of Illustrations and Tables
21 b/w illustrations, 18 illustrations in colour
Topics