Methods in Molecular Biology

RNA Detection and Visualization

Methods and Protocols

Editors: Gerst, Jeffrey E. (Ed.)

  • -Features methods and protocols relevant for both fixed and live cells from everything from bacteria to mammalian models
  • -Provides detailed techniques from established and up-and-coming experts in the field
  • -Includes vital notes on troubleshooting and avoiding known pitfalls for easier implementation of the methods
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-005-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-61779-004-1
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  • Usually dispatched within 3 to 5 business days.
About this book

With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions.  In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals.  For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, RNA Detection and Visualization: Methods and Protocols offers well-honed techniques in order to inspire researchers around the world to further our knowledge of the vital biological significance of RNA.

Table of contents (28 chapters)

  • Single Molecule Imaging of RNA In Situ

    Batish, Mona (et al.)

    Pages 3-13

  • FISH and Immunofluorescence Staining in Chlamydomonas

    Uniacke, James (et al.)

    Pages 15-29

  • High Resolution Fluorescent In Situ Hybridization in Drosophila

    Lécuyer, Eric

    Pages 31-47

  • Localization and Anchorage of Maternal mRNAs to Cortical Structures of Ascidian Eggs and Embryos Using High Resolution In Situ Hybridization

    Paix, Alexandre (et al.)

    Pages 49-70

  • Visualization of mRNA Localization in Xenopus Oocytes

    Gagnon, James A. (et al.)

    Pages 71-82

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-005-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-61779-004-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
RNA Detection and Visualization
Book Subtitle
Methods and Protocols
Editors
  • Jeffrey E. Gerst
Series Title
Methods in Molecular Biology
Series Volume
714
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-005-8
DOI
10.1007/978-1-61779-005-8
Hardcover ISBN
978-1-61779-004-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 497
Topics