Overview
- Editors:
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Teresa S. Hawley
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Medical Center, Flow Cytometry Core Facility, George Washington University, Washington, USA
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Robert G. Hawley
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Medical Center, Dept. Anatomy & Regenerative Biology, George Washington University, Washington, USA
- Features polychromatic, quantitative, and high throughput flow cytometry protocols applicable to basic biological research as well as diagnostic medicine
- Paves the way for flow cytometry's use in the study of systems biology
- Includes extensive expert notes to ensure optimal results
- Includes supplementary material: sn.pub/extras
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Table of contents (24 protocols)
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- Lili Wang, Adolfas K. Gaigalas, Ming Yan
Pages 53-65
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- Tione Buranda, Yang Wu, Larry A. Sklar
Pages 67-84
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- Henri C. van der Heyde, Irene Gramaglia
Pages 85-95
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- Shane Bruckner, Ling Wang, Ruiling Yuan, Perry Haaland, Amitabh Gaur
Pages 97-118
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- Joseph D. Tario Jr., Katharine A. Muirhead, Dalin Pan, Mark E. Munson, Paul K. Wallace
Pages 119-164
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- Patricia Lovelace, Holden T. Maecker
Pages 165-178
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- Peter O. Krutzik, Angelica Trejo, Kenneth R. Schulz, Garry P. Nolan
Pages 179-202
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- William G. Telford, Akira Komoriya, Beverly Z. Packard, C. Bruce Bagwell
Pages 203-227
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- James W. Jacobberger, R. Michael Sramkoski, Tammy Stefan
Pages 229-249
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- Ludovic Zimmerlin, Vera S. Donnenberg, Albert D. Donnenberg
Pages 251-273
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- Cornelis J. H. Pronk, David Bryder
Pages 275-293
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- Katherine R. Calvo, Catharine S. McCoy, Maryalice Stetler-Stevenson
Pages 295-316
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- Maurice R. G. O’Gorman, Joshua Zollett, Nicolas Bensen
Pages 317-335
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- Henri C. van der Heyde, Irene Gramaglia, Valéry Combes, Thaddeus C. George, Georges E. Grau
Pages 337-354
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- Rita L. Strack, Robert J. Keenan, Benjamin S. Glick
Pages 355-370
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- György Vereb, Péter Nagy, János Szöllo˝si
Pages 371-392
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- M. Raza Zaidi, Chi-Ping Day, Glenn Merlino
Pages 393-405
About this book
With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users.
Editors and Affiliations
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Medical Center, Flow Cytometry Core Facility, George Washington University, Washington, USA
Teresa S. Hawley
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Medical Center, Dept. Anatomy & Regenerative Biology, George Washington University, Washington, USA
Robert G. Hawley
