Methods in Molecular Biology

Signal Transduction Protocols

Editors: Dickson, Robert C., Mendenhall, Michael D. (Eds.)

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About this book

Carrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated Signal Transduction Protocols with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and eminently practical, Signal Transduction Protocols, 2nd ed. offers novice and experienced researchers alike an indispensable set of standardized, but readily adaptable, approaches for the assay of key signaling molecules-an invaluable tool for all laboratories newly entering the field, expanding the breadth of previous investigations, or seeking to improve an existing approach.

Reviews

Reviews of the first edition:
...this well-organized volume is clearly among the most viable protocol books published in this field and will turn out to be a helpful tool for many laboratories.-FEBS Letters

Table of contents (21 chapters)

  • Making Protein Immunoprecipitates

    Elion, Elaine A. (et al.)

    Pages 1-14

  • Signal Transduction Inhibitors in Cellular Function

    Fu, Maofu (et al.)

    Pages 15-36

  • Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets

    Kabuyama, Yukihito (et al.)

    Pages 37-49

  • A High-Throughput Mammalian Cell-Based Transient Transfection Assay

    Noonan, Daniel J. (et al.)

    Pages 51-65

  • Determining Protein Half-Lives

    Zhou, Pengbo

    Pages 67-77

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-59259-816-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $115.00
price for USA
  • ISBN 978-1-61737-448-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Signal Transduction Protocols
Editors
  • Robert C. Dickson
  • Michael D. Mendenhall
Series Title
Methods in Molecular Biology
Series Volume
284
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-816-8
DOI
10.1385/1592598161
Softcover ISBN
978-1-61737-448-7
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XII, 327
Topics