Methods in Molecular Biology

DNA'Protein Interactions

Principles and Protocols

Editors: Moss, Tom (Ed.)

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About this book

DNA-protein interactions are fundamental to understanding biological growth, development, differentiation, evolution, and disease, and are also increasingly important to the biotechnology industry. In DNA-Protein Interactions: Principles and Protocols, 2nd Edition, Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions, define their components, and determine the nature of their interactions and their manner of function, both in the cell and in the test tube. Each detailed method has been optimized and tested by a hands-on expert to ensure experimental success, and provides helpful notes on pitfalls to avoid, as well as alternate strategies. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. There are also vanguard methods for designing proteins to fit DNA, site-specific protein-directed DNA cleavage, and 2D semicrystalline arrays of nucleoproteins for structure determination.
Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to illuminate DNA-protein interactions.

Reviews

"The topics are organized in a sensible linear manner, starting with elementary protein-DNA binding assays such as filter binding and EMSA, leading to footprinting techniques for the identification of individual contacts and DNA distortion, through more complex crosslinking and spectroscopic methods, to the final chapters on crystallization and some highly specialized biophysical techniques such as force microscopy....this book will find favor with both the novice and the expert; the former will learn techniques that will be useful for many years whilst the latter will be inspired by discovering new applications." - Molecular Biotechnology

Buy this book

Spiral binding $115.00
price for USA
  • ISBN 978-0-89603-671-0
  • Free shipping for individuals worldwide
  • This title is currently reprinting. You can pre-order your copy now.
Softcover $115.00
price for USA
  • ISBN 978-1-61737-125-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.

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Bibliographic Information

Bibliographic Information
Book Title
DNA'Protein Interactions
Book Subtitle
Principles and Protocols
Editors
  • Tom Moss
Series Title
Methods in Molecular Biology
Series Volume
148
Copyright
2001
Publisher
Humana Press
Copyright Holder
Humana Press
Spiral binding ISBN
978-0-89603-671-0
Softcover ISBN
978-1-61737-125-7
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XVII, 638
Topics