Methods in Molecular Biology

3D Cell Culture

Methods and Protocols

Editors: Haycock, John (Ed.)

  • Features detailed methods for three dimensional cell culture, most notably in the formation of tissues for clinical purpose
  • Written by experts at engineering the reproduction of a variety of 3D cell culture models
  • Includes vital tips on troubleshooting and avoiding known protocol pitfalls
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-60761-984-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-60761-983-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-5727-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Developed for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Protocols, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose. Beginning with an overview of the biological and materials scaffold requirements for successfully creating 3D models, the book delves into topics such as general scaffold design and fabrication techniques, models for bone, skin, cartilage, nerve, bladder, and hair follicles, and chapters on bioreactor design, imaging, and stem cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text but also require techniques described using a standard, convenient format.

Reviews

From the reviews:

“This book is an excellent resource to help researchers find practical information to enable them to develop three-dimensional cell culture systems. … anyone who wishes to enter this field should be able to use this book to develop their own bespoke 3D system. In conclusion, I would recommend this book as being very useful to a wide audience … . will be essential for graduate students and postdocs as well as to investigators wishing to expand their work into 3D cell culture and tissue engineering.” (Julian B. Chaudhuri, ChemBioChem, Vol. 12, 2011)

“A necessary book for all who use cell culture. No other words can better tell the reader about this compendium of the most innovative culture techniques we hold to try to reach the ultimate goal of cell culture … . For those who wish to enter the field, some introductory reviews explain the present-day challenges in 3D culture while more detailed protocols talk about bioreactors and agarose constructs.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)


Table of contents (20 chapters)

  • 3D Cell Culture: A Review of Current Approaches and Techniques

    Haycock, John W.

    Pages 1-15

  • Scaffolds for Tissue Engineering and 3D Cell Culture

    Carletti, Eleonora (et al.)

    Pages 17-39

  • Tracking Nanoparticles in Three-Dimensional Tissue-Engineered Models Using Confocal Laser Scanning Microscopy

    Hearnden, Vanessa (et al.)

    Pages 41-51

  • Using Immuno-Scanning Electron Microscopy for the Observation of Focal Adhesion-substratum interactions at the Nano- and Microscale in S-Phase Cells

    Biggs, Manus J. P. (et al.)

    Pages 53-60

  • 3D Sample Preparation for Orthopaedic Tissue Engineering Bioreactors

    Cartmell, Sarah H. (et al.)

    Pages 61-76

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-60761-984-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-60761-983-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-5727-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
3D Cell Culture
Book Subtitle
Methods and Protocols
Editors
  • John Haycock
Series Title
Methods in Molecular Biology
Series Volume
695
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60761-984-0
DOI
10.1007/978-1-60761-984-0
Hardcover ISBN
978-1-60761-983-3
Softcover ISBN
978-1-4939-5727-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 343
Topics