Molecular Imaging

1. Front Matter

   Pages i-xi

   PDF

2. Imaging Biochemical Pathways and Molecular Events

   1. Front Matter

      Pages 1-1

      PDF

   2. Bioluminescence Resonance Energy Transfer (BRET) Imaging in Plant Seedlings and Mammalian Cells

      • Qiguang Xie*, Mohammed Soutto*, Xiaodong Xu*, Yunfei Zhang, Carl Hirschie Johnson

      Pages 3-28

   3. Luciferase Protein Complementation Assays for Bioluminescence Imaging of Cells and Mice

      • Gary D. Luker, Kathryn E. Luker

      Pages 29-43

   4. Hybrid Raman-Fluorescence Microscopy on Single Cells Using Quantum Dots

      • Henk-Jan van Manen, Cees Otto

      Pages 45-60

   5. Labeling of Mesenchymal Stem Cells with Bioconjugated Quantum Dots

      • Bhranti S. Shah, Jeremy J. Mao

      Pages 61-75

   6. Quantification of miRNA Abundance in Single Cells Using Locked Nucleic Acid-FISH and Enzyme-Labeled Fluorescence

      • Jing Lu, Andrew Tsourkas

      Pages 77-88

3. Imaging in Pre-clinical Settings

   1. Front Matter

      Pages 89-89

      PDF

   2. Imaging Fate of Stem Cells at a Cellular Resolution in the Brains of Mice

      • Khalid Shah

      Pages 91-101

   3. Magnetic Resonance Imaging of Brain Inflammation Using Microparticles of Iron Oxide

      • Martina A. McAteer, Constantin von Zur Muhlen, Daniel C. Anthony, Nicola R. Sibson, Robin P. Choudhury

      Pages 103-115

   4. Optical Characterization of Arterial Apoptosis

      • Maarten F. Corsten, Abdelkader Bennaghmouch

      Pages 117-129

   5. Intravital Fluorescence Microscopic Molecular Imaging of Atherosclerosis

      • Farouc A. Jaffer

      Pages 131-140

   6. MR Imaging of Transplanted Stem Cells in Myocardial Infarction

      • Dara L. Kraitchman, Dorota A. Kedziorek, Jeff W.M. Bulte

      Pages 141-152

   7. In Vivo Imaging of the Dynamics of Different Variants of EGFR in Glioblastomas

      • Khalid Shah

      Pages 153-164

   8. Fluorescence Lifetime-Based Optical Molecular Imaging

      • Anand T.N. Kumar

      Pages 165-180

4. Imaging in Clinical Settings

   1. Front Matter

      Pages 181-181

      PDF

   2. PET Imaging of αvβ3 Expression in Cancer Patients

      • Ambros J. Beer, Markus Schwaiger

      Pages 183-200

   3. Quantitative Approaches to Amyloid Imaging

      • Victor L. Villemagne, Graeme O’Keefe, Rachel S. Mulligan, Christopher C. Rowe

      Pages 201-225

   4. Molecular Imaging of Myocardial Remodeling After Infarction

      • Johan W.H. Verjans, Susanne W.M. van de Borne, Leonard Hofstra, Jagat Narula

      Pages 227-235

   5. Dual-Radionuclide Brain SPECT for the Differential Diagnosis of Parkinsonism

      • Georges El Fakhri, Jinsong Ouyang

      Pages 237-246

In recent years, molecular imaging techniques have grown to be invaluable tools for molecular biology research and, to a more modest extent, clinical medicine. In Molecular Imaging: Methods and Protocols, expert researchers explore the latest advances in the field, describing a rich variety of practical procedures and methods for diverging imaging technologies. Chapters are divided into sections that cover the imaging of basic molecular and biochemical events, imaging in pre-clinical settings, and imaging in clinical settings. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and ground-breaking, Molecular Imaging: Methods and Protocols is an essential handbook for students, established practitioners, and research fellows alike.

• Bioluminescence
• Magnetic resonance imaging
• Magnetic resonance spectroscopy
• Radio-scintigraphic modalities
• Visible spectrum fluorescence

From the reviews:

“This handbook describes a rich variety of practical procedures and methods for diverging imaging technologies. … This sufficiently detailed handbook is essential for students, research fellows, and established practitioners from different fields to become familiar with molecular imaging and incorporate it into their work. I highly recommend the book to trainees and practitioners in nuclear medicine and radiology with research interest in molecular imaging.” (E. Edmund Kim, The Journal of Nuclear Medicine, Vol. 53 (1), January, 2012)

“The book covers many aspects of molecular imaging at the cellular and subcellular level and within intact living organisms. The techniques are well described and protocols are provided in detail, with a lot of technical specifications, also concerning instrumentation settings and the software used. All this information is of fundamental importance for imaging measurements and is seldom reported in the scientific literature. …. can be recommended to students, research fellows, and technicians working or planning to work with molecular imaging techniques.” (Elisa Michelini, Analytical and Bioanalytical Chemistry, Vol. 403, 2012)

“Excellently presented, this book on molecular imaging provides valuable information on practical procedures and methods for divergent technologies for imaging of basic molecular and biochemical events in addition to imaging in preclinical and clinical settings. … The book is intended for students, established practitioners, and research fellows from other fields who want to become familiar with molecular imaging … . graduate students, practicing radiologists and imaging experts, research fellows, and anyone interested in molecular imaging will find this book valuable.” (Omer Iqbal, Doody's Book Reviews, March, 2011)

• Massachusetts General Hospital, Department of Radiology and Neurology, Harvard Medical School, Charlestown, USA

  Khalid Shah

Policies and ethics