Methods in Molecular Biology

Metagenomics

Methods and Protocols

Editors: Streit, Wolfgang, Daniel, Rolf (Eds.)

  • Introduces basic methods commonly used in laboratories that have a strong background in microbial metagenomics
  • Provides a diverse range of screening protocols for metagenome libraries
  • Examines expert tips on troubleshooting and avoiding troublesome pitfalls
  • Covers a wide range of metagenome function- and sequence-based screening technologies
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-60761-823-2
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  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-60761-822-5
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About this book

Metagenomics has proven to be a powerful tool for exploring the ecology, metabolic profiling, and comparison of complex microbial communities as well as its important applications in the mining of metagenomes for genes encoding novel biocatalysts and drug molecules for bioindustries. In Metagenomics: Methods and Protocols, expert researches provide an overview and introduction to basic methods commonly used in laboratories that have a strong background in microbial metagenomics. The book attempts to address all of the working steps involved in this crucial field, beginning with DNA isolation from soils and marine samples and continuing with the construction and screening of libraries, along with key advise involving bioinformatic tools available to analyze large metagenomic sequence data sets. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metagenomics: Methods and Protocols serves as a very complete guide to available screening protocols for all major biocatalysts in order to allow for the easy setup of these screens in any microbiology lab.

Table of contents (24 chapters)

  • Molecular Methods to Study Complex Microbial Communities

    Vieites, José M. (et al.)

    Pages 1-37

  • Construction of Small-Insert and Large-Insert Metagenomic Libraries

    Simon, Carola (et al.)

    Pages 39-50

  • Construction and Screening of Marine Metagenomic Libraries

    Weiland, Nancy (et al.)

    Pages 51-65

  • Metagenomic Analysis of Isotopically Enriched DNA

    Chen, Yin (et al.)

    Pages 67-75

  • Wide Host-Range Cloning for Functional Metagenomics

    Wexler, Margaret (et al.)

    Pages 77-96

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-60761-823-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-60761-822-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Metagenomics
Book Subtitle
Methods and Protocols
Editors
  • Wolfgang Streit
  • Rolf Daniel
Series Title
Methods in Molecular Biology
Series Volume
668
Copyright
2010
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60761-823-2
DOI
10.1007/978-1-60761-823-2
Hardcover ISBN
978-1-60761-822-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 341
Topics