Methods in Molecular Biology

Fluorescence in situ Hybridization (FISH)

Protocols and Applications

Editors: Bridger, Joanna M., Morris, Keith (Eds.)

  • Contains a plethora of modern adaptations to the FISH methodology
  • Will benefit both clinical and research laboratories
  • Informs how to achieve and recognize quality in the different FISH methodologies
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-60761-789-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-60761-788-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The design simplicity and cost-effectiveness of the early Fluorescence in situ Hybridization (FISH) protocols, combined with the significant acceleration of discoveries in related technical areas like fluorescence microscopy, digital imaging, and nucleic acid technology have prompted the diversification of the original technique into an outstanding number of imaginative and useful applications, thus promoting its expansion into different areas of basic and applied research in the post-genomic era. In Fluorescence in situ Hybridization (FISH): Protocols and Applications, experts in the field portray the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of various applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology and evolutionary studies. The book is divided into four convenient sections covering the core techniques, technical advancements and novel adaptations, applications for human genetics and medicine, as well as protocols for model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Fluorescence in situ Hybridization (FISH): Protocols and Applications aims to aid scientists from across the world in applying this tried-and-true scientific technique to their own lab’s current research.

Reviews

From the reviews:

“Fluorescence in situ hybridization (FISH) has been widely adopted to enable the study of uncultured target cells. This book shows many more applications in cytology. Relatively few chapters are devoted directly to microbiological applications; the strong theme of the book describes methods targeting chromosomes. … Protocols on 3-D FISH may have useful material for microbiologists, e.g. studies on biofilms. … I would suggest this is for microbiological libraries … .” (Jonathan Porter, Microbiology Today, November, 2011)

Table of contents (34 chapters)

  • Fluorescence in situ Hybridization (FISH), Basic Principles and Methodology

    Garimberti, Elisa (et al.)

    Pages 3-20

  • Fluorescence In Situ Hybridization on DNA Halo Preparations and Extended Chromatin Fibres

    Elcock, Lauren S. (et al.)

    Pages 21-31

  • Detection of Nascent RNA Transcripts by Fluorescence In Situ Hybridization

    Brown, Jill M. (et al.)

    Pages 33-50

  • Fluorescence In Situ Hybridization Analysis of Formalin Fixed Paraffin Embedded Tissues, Including Tissue Microarrays

    Summersgill, Brenda M. (et al.)

    Pages 51-70

  • Fluorescence in situ Hybridization (FISH) on Tissue Cryosections

    Solovei, Irina

    Pages 71-82

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-60761-789-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-60761-788-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Fluorescence in situ Hybridization (FISH)
Book Subtitle
Protocols and Applications
Editors
  • Joanna M. Bridger
  • Keith Morris
Series Title
Methods in Molecular Biology
Series Volume
659
Copyright
2010
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60761-789-1
DOI
10.1007/978-1-60761-789-1
Hardcover ISBN
978-1-60761-788-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 451
Topics