Fluorescence in situ Hybridization (FISH)

1. Front Matter

   Pages i-xiv

   PDF

2. Core Techniques

   1. Front Matter

      Pages 1-1

      PDF

   2. Fluorescence in situ Hybridization (FISH), Basic Principles and Methodology

      • Elisa Garimberti, Sabrina Tosi

      Pages 3-20

   3. Fluorescence In Situ Hybridization on DNA Halo Preparations and Extended Chromatin Fibres

      • Lauren S. Elcock, Joanna M. Bridger

      Pages 21-31

   4. Detection of Nascent RNA Transcripts by Fluorescence In Situ Hybridization

      • Jill M. Brown, Veronica J. Buckle

      Pages 33-50

   5. Fluorescence In Situ Hybridization Analysis of Formalin Fixed Paraffin Embedded Tissues, Including Tissue Microarrays

      • Brenda M. Summersgill, Janet M. Shipley

      Pages 51-70

   6. Fluorescence in situ Hybridization (FISH) on Tissue Cryosections

      • Irina Solovei

      Pages 71-82

   7. Multiplex Fluorescence in situ Hybridization (M-FISH)

      • Rhona Anderson

      Pages 83-97

   8. Optical Mapping of Protein–DNA Complexes on Chromatin Fibers

      • Beth A. Sullivan

      Pages 99-115

   9. 3D-FISH on Cultured Cells Combined with Immunostaining

      • Irina Solovei, Marion Cremer

      Pages 117-126

3. Technical Advancements and Novel Adaptations

   1. Front Matter

      Pages 127-127

      PDF

   2. The Comet-FISH Assay for the Analysis of DNA Damage and Repair

      • Graciela Spivak

      Pages 129-145

   3. Direct In Situ Hybridization with Oligonucleotide Functionalized Quantum Dot Probes

      • Laurent A. Bentolila

      Pages 147-163

   4. LNA-FISH for Detection of MicroRNAs in Frozen Sections

      • Asli N. Silahtaroglu

      Pages 165-171

   5. Chromosome Orientation Fluorescence In Situ Hybridization or Strand-Specific FISH

      • Susan M. Bailey, Eli S. Williams, Michael N. Cornforth, Edwin H. Goodwin

      Pages 173-183

   6. Combinatorial Oligo FISH: Directed Labeling of Specific Genome Domains in Differentially Fixed Cell Material and Live Cells

      • Eberhard Schmitt, Jutta Schwarz-Finsterle, Stefan Stein, Carmen Boxler, Patrick Müller, Andriy Mokhir et al.

      Pages 185-202

   7. Simultaneous Visualization of FISH Signals and Bromo-deoxyuridine Incorporation by Formamide-Free DNA Denaturation

      • Daniela Moralli, Zoia L. Monaco

      Pages 203-218

   8. CryoFISH: Fluorescence In Situ Hybridization on Ultrathin Cryosections

      • Sheila Q. Xie, Liron-Mark Lavitas, Ana Pombo

      Pages 219-230

   9. Characterization of Chromosomal Rearrangements Using Multicolor-Banding (MCB/m-band)

      • Thomas Liehr, Anja Weise, Sophie Hinreiner, Hasmik Mkrtchyan, Kristin Mrasek, Nadezda Kosyakova

      Pages 231-238

   10. Visualizing Nucleic Acids in Living Cells by Fluorescence In Vivo Hybridization

       • Joop Wiegant, Anneke K. Brouwer, Hans J. Tanke, Roeland W. Dirks

       Pages 239-246

Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.

• Chromosom
• DNA
• DNA halos
• FISH
• Fluorescence microscopy
• Fluoreszenz in situ-Hybridisierung
• Genetic-based diagnostics & prognosis
• Microarray
• Model organisms
• Molecular cytogenetics
• Translational FISH
• genetics
• hybridization
• molecular biology
• tissue
• cytogenetics

From the reviews:

“Fluorescence in situ hybridization (FISH) has been widely adopted to enable the study of uncultured target cells. This book shows many more applications in cytology. Relatively few chapters are devoted directly to microbiological applications; the strong theme of the book describes methods targeting chromosomes. … Protocols on 3-D FISH may have useful material for microbiologists, e.g. studies on biofilms. … I would suggest this is for microbiological libraries … .” (Jonathan Porter, Microbiology Today, November, 2011)

• School of Health Sciences & Social Care, Div. Biosciences, Brunel University, Uxbridge, United Kingdom

  Joanna M. Bridger

• , Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom

  Emanuela V. Volpi

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