Methods in Molecular Biology

Transcription Factors

Methods and Protocols

Editors: Higgins, Paul J. (Ed.)

  • The ‘methods’ chapters are preceded by several excellent reviews that highlight the role of post-translational modifications and subcellular trafficking in transcription factor
  • Includes new techniques for assessing kinase targeting of specific transcription factors, sites of phosphorylation and the involved signaling pathways
  • Describes novel molecular and elegant microscopic imaging-based procedures to evaluate nuclear translocation and export in living cells
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eBook $139.00
price for USA (gross)
  • ISBN 978-1-60761-738-9
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  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA
  • ISBN 978-1-60761-737-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

In the last few years, significant breakthroughs in transcription research expanded our appreciation for the complexity of molecular controls on gene expression in mammalian cells. In Transcription Factors: Methods and Protocols, experts in the field describe state-of-the-art approaches that investigators can use to probe critical mechanisms underlying transcription factor nuclear-cytoplasmic trafficking as well as to assess the functional impact of post-translational modifications on transcription factor function. The chapters are written by prominent scientists, many of whom developed these methods, and highlight protocols that focus on specific transcription factor family members with particular relevance to human disease. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and current, Transcription Factors: Methods and Protocols compiles the latest techniques for elucidating controls on transcription factor intracellular localization and activity, and consequently is unlike any other methods-based text on transcriptional regulation today. 

Table of contents (24 chapters)

  • A Review of Post-translational Modifications and Subcellular Localization of Ets Transcription Factors: Possible Connection with Cancer and Involvement in the Hypoxic Response

    Charlot, Céline (et al.)

    Pages 3-30

  • Regulation of Transcription Factor Function by Targeted Protein Degradation: An Overview Focusing on p53, c-Myc, and c-Jun

    Westermarck, Jukka

    Pages 31-36

  • Review of Molecular Mechanisms Involved in the Activation of the Nrf2-ARE Signaling Pathway by Chemopreventive Agents

    Giudice, Aldo (et al.)

    Pages 37-74

  • Subnuclear Localization and Intranuclear Trafficking of Transcription Factors

    Zaidi, Sayyed K. (et al.)

    Pages 77-93

  • Analysis of Ligand-Dependent Nuclear Accumulation of Smads in TGF-β Signaling

    Chapnick, Douglas A. (et al.)

    Pages 95-111

Buy this book

eBook $139.00
price for USA (gross)
  • ISBN 978-1-60761-738-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA
  • ISBN 978-1-60761-737-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Transcription Factors
Book Subtitle
Methods and Protocols
Editors
  • Paul J. Higgins
Series Title
Methods in Molecular Biology
Series Volume
647
Copyright
2010
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60761-738-9
DOI
10.1007/978-1-60761-738-9
Hardcover ISBN
978-1-60761-737-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 426
Topics