Happy Holidays! Over 120,000 eBooks at just 19.99 each— Pick a favorite today

Methods in Molecular Biology

Hepatocytes

Methods and Protocols

Editors: Maurel, Patrick (Ed.)

  • Presents a comprehensive collection of cutting-edge methods for research on hepatocytes and stem cells
  • Supplies core methods for human hepatocyte isolation, culture, differentiation, cryopreservation, and transplantation
  • Examines the state of the art of liver development
  • Provides methods for generating hepatocytes from stem cells of different sources
  • Details technical aspects of hepatocyte use in drug metabolism and toxicology as well as technical aspects of hepatocyte use in endogenous metabolism and virology
  • Features chimeric mice with humanized liver and bioartificial liver support and liver biotherapy
see more benefits

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-60761-688-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA
  • ISBN 978-1-60761-687-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.00
price for USA
  • ISBN 978-1-4939-5716-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Hepatocytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepatocytes: Methods and Protocols, expert researchers provide the reader with methods, technical protocols, and review chapters focusing on selected areas of hepatocyte biology including isolation, culture, differentiation and stem cells, and hepatocyte use in clinical, basic, and applied research. With a specific emphasis on human hepatocytes, the volume presents chapters covering subjects including hepatocyte culture models, cryopreservation methods, differentiation assessment, liver ontogenesis, production of hepatocytes from stem cells, drug/xenobiotic metabolism, toxicity and transport, bile acid and blood coagulation factor production, infection by HBV and HCV, humanized animals, biortificial liver devices, hepatocyte transplantation. As a volume in the highly successful Methods in Molecular Biology™ series, protocol chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Hepatocytes: Methods and Protocols will be useful to all those who are currently using or planning to use human, or animal, hepatocytes to investigate any aspect of liver physiopathology or who are interested in liver development or liver stem cells and liver biotherapy.

Reviews

From the reviews:

“Hepatocytes presents a well matched mix of theory and practice. … A very welcome general theme throughout the book are the detailed practical aspects. Some chapters might almost be training manuals in this respect. … I found the book very hard to criticise To me, it was the ideal textbook … . Moreover each chapter was written by contributors that dripped with experience. … the quality of the text is so outstanding.” (Graham Lappin, BTS Newsletter, Issue 37, Winter, 2010)

“Eighty authors have collaborated to construct 29 chapters … . the quality of the paper, the layout and print size, and the overall construction of the book are excellent. The 553 pages are abundantly illustrated and annotated with 1591 references followed by a 19-page index … . The work is a worthy and elegant addition to their ‘Methods in Molecular Biology’ series and would be equally at home, and useful, around the laboratory, on the office shelf or in a reference library.” (S. C. Mitchell, ISSX Newsletter, February, 2011)


Table of contents (29 chapters)

  • General Review on In Vitro Hepatocyte Models and Their Applications

    Guguen-Guillouzo, Christiane (et al.)

    Pages 1-40

  • Human Foetal Hepatocytes: Isolation, Characterization, and Transplantation

    Weber, Anne (et al.)

    Pages 41-55

  • Isolation and Culture of Primary Hepatocytes from Resected Human Liver Tissue

    LeCluyse, Edward L. (et al.)

    Pages 57-82

  • Optimisation of the Cryopreservation of Primary Hepatocytes

    Hewitt, Nicola J.

    Pages 83-105

  • Cryopreservation of Human Hepatocytes for Clinical Use

    Hughes, Robin D. (et al.)

    Pages 107-113

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-60761-688-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA
  • ISBN 978-1-60761-687-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.00
price for USA
  • ISBN 978-1-4939-5716-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Hepatocytes
Book Subtitle
Methods and Protocols
Editors
  • Patrick Maurel
Series Title
Methods in Molecular Biology
Series Volume
640
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60761-688-7
DOI
10.1007/978-1-60761-688-7
Hardcover ISBN
978-1-60761-687-0
Softcover ISBN
978-1-4939-5716-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIX, 553
Topics