Methods in Molecular Biology

Peptidomics

Methods and Protocols

Editors: Soloviev, Mikhail (Ed.)

  • Collective effort by nearly 70 scientists from over 20 leading laboratories worldwide to produce an all-inclusive yet concise practical manual to peptidomics
  • Complete: Includes all peptidomic methods in a single volume
  • Comprehensive: Covers a wide range of species from bacterial to man
  • Convenient: Provides step-by-step laboratory protocols with troubleshooting advice
  • Captivating: Supplies the best practical introduction to the fascinating world of peptides
  • Creative: Impresses with the thoroughness of the methods and the ingenuity of the experimental design
see more benefits

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-60761-535-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-60761-534-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Despite being known and studied for years, peptides have never before attracted enough attention to necessitate the invention of the term "peptidomics" in order to specify the study of the complement of peptides from a cell, organelle, tissue or organism. In Peptidomics: Methods and Protocols, expert researchers present a comprehensive range of analytical techniques for the analysis of the peptide contents of complex biological samples with an emphasis often on higher throughput techniques, suitable for the analysis of large numbers of peptides typically present in the peptidomes. Encompassing a number of species ranging from bacteria to man, the methods presented intensively cover topics such as organism handling, tissue and organ dissection, cellular and subcellular fractionation, peptide extraction, fractionation and purification, structural characterization, molecular cloning, and sequence analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Peptidomics: Methods and Protocols brings this ten year old field fully up-to-date in order to inspire novices and experts alike with the easy-to-follow practical advice needed to set up and carry out analysis of the peptide contents of complex biological samples.

Reviews

From the reviews:

“Peptidomics–Methods and Protocols focuses on high-throughput techniques for the analysis of peptidomes from a wide range of biological systems … . The procedures covered in this book encompass a number of model and non-model organisms, and most chapters have been written by distinguished researchers who have made major contributions to their respective fields. Peptidomics–Methods and Protocols is an indispensable reference book for specialists, but also an introductory work for newcomers to the rapidly developing discipline of peptidomics.”­­­ (Juan J. Calvete, Analytical and Bioanalytical Chemistry, Vol. 398, September, 2010)

“The editor and co-authors of this volume must be congratulated on a comprehensive collection of very diverse peptidomics protocols that serve as an excellent introduction for a novice in this field. At the same time, the book provides the expert researcher with highly detailed experimental procedures and practical advice for the analysis of complex mixtures of peptides from a variety of sources. I warmly recommend this book to anyone interested in the field of peptidomics.” (Christian A. Olsen, ChemBioChem, Vol. 11, August, 2010)


Table of contents (26 chapters)

  • Peptidomics: Divide et Impera

    Soloviev, Mikhail

    Pages 3-9

  • Performing Comparative Peptidomics Analyses of Salmonella from Different Growth Conditions

    Adkins, Joshua N. (et al.)

    Pages 13-27

  • Approaches to Identify Endogenous Peptides in the Soil Nematode Caenorhabditis elegans

    Husson, Steven J. (et al.)

    Pages 29-47

  • Mass Spectrometric Analysis of Molluscan Neuropeptides

    Li, Ka Wan (et al.)

    Pages 49-56

  • Monitoring Neuropeptides In Vivo via Microdialysis and Mass Spectrometry

    Behrens, Heidi L. (et al.)

    Pages 57-73

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-60761-535-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-60761-534-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Peptidomics
Book Subtitle
Methods and Protocols
Editors
  • Mikhail Soloviev
Series Title
Methods in Molecular Biology
Series Volume
615
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60761-535-4
DOI
10.1007/978-1-60761-535-4
Hardcover ISBN
978-1-60761-534-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 395
Topics