Methods in Molecular Biology

Dendritic Cell Protocols

Editors: Naik, Shalin H. (Ed.)

  • Provides step-by-step procedures for DC isolation from rodent and human tissue, as well as easy in vitro generation of equivalent cell types
  • Explores diverse assays to measure DC function in the context of tolerance, autoimmune disease, allergy, and immunity to viral and bacterial infection
  • All assays used currently by leading experts in the field for high impact studies
  • Offers a digestible introduction to DC function and development, covering both humans and rodents
  • Includes techniques that are cutting edge and state-of-the-art in the field of DC biology
  • Contains a comprehensive list of models to test the effect that your favourite gene or immunological model has on DC development or function
  • Helps readers to generate a particular specialized DC subtype in vitro saving time with greater focus on a biologically relevant system
  • Examines isolating DC from almost all organs of the mouse
  • Aids in the identification of the myriad subtypes in human and mice for further study
  • Provides a standardized model for the use of human DCs in clinical trials
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-60761-421-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-60761-420-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-61779-676-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, Dendritic Cell Protocols, Second Edition presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans. The first section of the volume involving humans explores topics such as the isolation of blood DC subtypes, primary skin Langerhans cells, and the generation of gene-manipulated human DCs with the inclusion of more clinically relevant methods as well, while the second section involving rodent models delves into DC and precursor generation in vitro, isolation ex vivo, disease models, as well as DC functions and properties. Written in the highly successful Methods in Molecular Biology™ series style, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Dendritic Cell Protocols, Second Edition aims to become a bench-side handbook for both beginners and experts in the field of DC research and a long-term reference for some of the most popular methods put forward by those who lead the field.

Reviews

From the reviews of the second edition:

“Written for principal investigators and their students, postdocs, and staff, providing very practical step-by-step instructions on how to work with DC. … Overall, this book provides a nice compilation of methods for studying dendritic cells. The book will be particularly useful for those studying murine DC, but will also provide some useful … information for those initiating human DC work. … this updated book, which is unique in the field, will make a nice addition to personal and institutional biomedical research libraries.” (Patricia Fitzgerald-Bocarsly, Doody’s Review Service, July, 2010)

Table of contents (28 chapters)

  • Review of Human DC Subtypes

    Ju, Xinsheng (et al.)

    Pages 3-20

  • Review of Murine Dendritic Cells: Types, Location, and Development

    Miloud, Tewfik (et al.)

    Pages 21-42

  • Isolation of Human Blood DC Subtypes

    Kassianos, Andrew J. (et al.)

    Pages 45-54

  • Isolation of Immature Primary Langerhans Cells from Human Epidermal Skin

    Jong, Marein A.W.P. (et al.)

    Pages 55-65

  • Isolation and In Vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic Cells

    Schotte, Remko (et al.)

    Pages 67-85

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-60761-421-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-60761-420-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-61779-676-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Dendritic Cell Protocols
Editors
  • Shalin H. Naik
Series Title
Methods in Molecular Biology
Series Volume
595
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60761-421-0
DOI
10.1007/978-1-60761-421-0
Hardcover ISBN
978-1-60761-420-3
Softcover ISBN
978-1-61779-676-0
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XVIII, 446
Number of Illustrations and Tables
60 b/w illustrations, 6 illustrations in colour
Additional Information
Originally published as volume 64 in the series: Methods in Molecular Medicine
Topics