Methods in Molecular Biology

Live Cell Imaging

Methods and Protocols

Editors: Papkovsky, Dmitri (Ed.)

  • Provides an easily accessible reference volume for live cell imaging
  • Written by leading researchers in the field
  • Details the analysis of a number of key biological parameters and processes on the scale of a single cell
  • Covers a broad range of imaging techniques, probes, assay formats, and applications
  • Presents a systematic approach to setting up imaging assays and conducting the experiments with live cells
  • Serves as a comprehensive guide to developing, running, and analyzing various parameters of cellular function
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-60761-404-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-60761-403-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable.  In Live Cell Imaging: Methods and Protocols, a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by representative examples and case studies covering different aspects of the most current methodology. By examining a range of state-of-the-art protocols extensively validated in complex biological studies, this volume highlights new experimental and instrumental opportunities and helps researchers to select appropriate imaging methods for their specific biological questions and measurement tasks.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Live Cell Imaging: Methods and Protocols promises to contribute greatly to the further development and dissemination of this fundamentally important technology which spans across many disciplines including molecular and cell biology, chemistry, physics, optics, engineering, cell physiology, and medicine.

Table of contents (21 chapters)

  • Instrumentation for Live-Cell Imaging and Main Formats

    Oheim, Martin

    Pages 3-16

  • Labels and Probes for Live Cell Imaging: Overview and Selection Guide

    A. Hilderbrand, Scott

    Pages 17-45

  • Live Cell Imaging: An Industrial Perspective

    McCann, Terry

    Pages 47-66

  • Design of Fluorescent Fusion Protein Probes

    Pham, Elizabeth (et al.)

    Pages 69-91

  • Synthetic Fluorescent Probes for Imaging of Peroxynitrite and Hypochlorous Acid in Living Cells

    Yang, Dan (et al.)

    Pages 93-103

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-60761-404-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-60761-403-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Live Cell Imaging
Book Subtitle
Methods and Protocols
Editors
  • Dmitri Papkovsky
Series Title
Methods in Molecular Biology
Series Volume
591
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60761-404-3
DOI
10.1007/978-1-60761-404-3
Hardcover ISBN
978-1-60761-403-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 367
Topics