Methods in Molecular Biology

Human Embryonic Stem Cell Protocols

Editors: Turksen, Kursad (Ed.)

  • Comprises a comprehensive guide to isolate culture and differentiate human embryonic stem cells in vitro
  • Provides easily followed detailed protocols
  • Covers numerous essential methods for culturing and analyzing human embryonic stem cells
  • Brings together different approaches to maintaining and differentiating human embryonic stem cells
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eBook $139.00
price for USA (gross)
  • ISBN 978-1-60761-369-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-60761-368-8
  • Free shipping for individuals worldwide
  • Online orders shipping within 2-3 days.
Softcover $179.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-6107-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, new freedoms in regard to using them for research has allowed interest to remain high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, Second Edition, internationally respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. As a volume of the Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols, Second Edition offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today.

Reviews

From the reviews of the second edition:

“Provides step-by-step protocols for the culture and directed differentiation of human embryonic stem cells. … written for graduate students, postdoctoral students, and basic science researchers … . valued by biomedical scientists and clinicians interested in tissue engineering. … The chapters are well written … . provide readers with a great resource that will be the standard of excellence in this field for years to come. … I recommend this book highly for all research scientists interested in cell culture and embryonic stem cell biology.” (Bruce A. Fenderson, Doody’s Review Service, July, 2010)

Table of contents (23 chapters)

  • Human Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells (ESC)

    Sathananthan, A. Henry (et al.)

    Pages 1-20

  • Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Embryos

    Peura, Teija T. (et al.)

    Pages 21-54

  • Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts

    Escobedo-Lucea, Carmen (et al.)

    Pages 55-69

  • In Vitro Neural Differentiation of Human Embryonic Stem Cells Using a Low-Density Mouse Embryonic Fibroblast Feeder Protocol

    Ozolek, John A. (et al.)

    Pages 71-95

  • Optimization of Physiological Xenofree Molecularly Defined Media and Matrices to Maintain Human Embryonic Stem Cell Pluripotency

    Peiffer, Isabelle (et al.)

    Pages 97-108

Buy this book

eBook $139.00
price for USA (gross)
  • ISBN 978-1-60761-369-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-60761-368-8
  • Free shipping for individuals worldwide
  • Online orders shipping within 2-3 days.
Softcover $179.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-6107-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Human Embryonic Stem Cell Protocols
Editors
  • Kursad Turksen
Series Title
Methods in Molecular Biology
Series Volume
584
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60761-369-5
DOI
10.1007/978-1-60761-369-5
Hardcover ISBN
978-1-60761-368-8
Softcover ISBN
978-1-4939-6107-8
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XII, 455
Additional Information
Originally pubblished as volume 331 in series: Methods in Molecular Biology
Topics