Methods in Molecular Biology

Lipidomics

Volume 2: Methods and Protocols

Editors: Armstrong, Donald (Ed.)

  • Regarding Volumes 1 and 2:
  • Contains in-depth guides to isolation and processing of lipid samples, probes, lipid profiling, signaling, transport, and mapping
  • Provides examples utilizing key model systems
  • Presents oxidized lipids obtained from in vitro and in vivo biological material, redox platforms, high throughput systems, microscopy and imaging, and computer simulation
  • Serves as a comprehensive guide to technical applications
  • Explores bioinformatics strategy and statistical models for standardization of lipids
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-60761-325-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-60761-324-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-61779-655-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics. These studies range from simple systems, such as in yeast, to complex biological models. Samples used in these studies include: mitochondria, microsomes, endosomal membranes, RBS and plasma membranes, vesicles, caveolae, liposomes, macrophages, meibum, brain, microglia, retina, adipose tissue, keratinocytes, pulmonary surfactant, and hepatic cultures.  Volume 2 focuses on oxidized lipids, trafficking and profiling, software, bioinformatics, and biostatistics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include useful introductions to their respective topics, lists of the necessary equipment and materials, step-by-step, readily reproducible laboratory protocols, and authoritative notes on troubleshooting and avoiding common pitfalls.

Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.

Table of contents (20 chapters)

  • F2-Isoprostanes: Sensitive Biomarkers of Oxidative Stress In Vitro and In Vivo: A Gas Chromatography-Mass Spectrometric Approach

    Wiswedel, Ingrid

    Pages 3-16

  • Volatile Oxylipins and Related Compounds Formed Under Stress in Plants

    Matsui, Kenji (et al.)

    Pages 17-28

  • Quantification of Lysophosphatidylcholine Species by High-Throughput Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS)

    Liebisch, Gerhard (et al.)

    Pages 29-37

  • Use of Lipidomics for Analyzing Glycerolipid and Cholesteryl Ester Oxidation by Gas Chromatography, HPLC, and On-line MS

    Kuksis, Arnis (et al.)

    Pages 39-91

  • Lipid Raft-Redox Signaling Platforms in Plasma Membrane

    Yi, Fan (et al.)

    Pages 93-107

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-60761-325-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-60761-324-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-61779-655-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Lipidomics
Book Subtitle
Volume 2: Methods and Protocols
Editors
  • Donald Armstrong
Series Title
Methods in Molecular Biology
Series Volume
580
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60761-325-1
DOI
10.1007/978-1-60761-325-1
Hardcover ISBN
978-1-60761-324-4
Softcover ISBN
978-1-61779-655-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 390
Number of Illustrations and Tables
105 b/w illustrations, 5 illustrations in colour
Topics