Springer Protocols Handbooks

Protocols for Neural Cell Culture

Editors: Doering, Laurie C. (Ed.)

  • Includes detailed protocols involving embryonic and adult stem cells, neuronal and glial preps, human and rodent cells, cellular assays, precursor cell expansion
  • Serves as an easy-to-use reference for neuroscientists to establish tissue culture preps of neural tissue to answer specific research questions
  • Provides the neuroscience community with procedures to isolate and culture the desired cell type from the fetal or adult nervous system
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-60761-292-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $169.00
price for USA
  • ISBN 978-1-60761-291-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies.  The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience.  While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death.  With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results.

Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the  previous editions’ tradition of exceptional quality and thorough coverage in tissue culture protocols for today’s neuroscience in order to inspire researchers in the field to further explore this imperative area of study.

Table of contents (23 chapters)

  • Neurosphere and Neural Colony-Forming Cell Assays

    Louis, Sharon A. (et al.)

    Pages 1-28

  • Directed Neuronal Differentiation of Embryonic and Adult-Derived Neurosphere Cells

    Costa, Marcos R. (et al.)

    Pages 29-49

  • Culture and Differentiation of Human Neural Stem Cells

    Shin, Soojung (et al.)

    Pages 51-73

  • Neural Differentiation of Human Embryonic Stem Cells

    Dottori, Mirella (et al.)

    Pages 75-86

  • Isolation and Culture of Primary Human CNS Neural Cells

    Blain, Manon (et al.)

    Pages 87-104

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-60761-292-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Softcover $169.00
price for USA
  • ISBN 978-1-60761-291-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protocols for Neural Cell Culture
Editors
  • Laurie C. Doering
Series Title
Springer Protocols Handbooks
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60761-292-6
DOI
10.1007/978-1-60761-292-6
Softcover ISBN
978-1-60761-291-9
Series ISSN
1949-2448
Edition Number
4
Number of Pages
XII, 400
Number of Illustrations and Tables
71 b/w illustrations, 14 illustrations in colour
Topics