Overview
- Provides an easily accessible reference for a range of biochemical and cell-based methods to study nuclear receptor function
- Clear step-by-step comprehensive guides written by experienced researchers in the field
- Covers cutting-edge methodologies, including ChIP-on-chip, siRNA gene silencing and tissues selective gene knock-outs
- Details analysis from isolated receptor proteins through to cellular and tissue function
- Presents experimental approaches, including the use of model biological systems and high-throughput screening, that can be applied to translational research
Part of the book series: Methods in Molecular Biology (MIMB, volume 505)
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Table of contents (15 protocols)
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Front Matter
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Introduction
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Nuclear Receptor—Co-Regulatory Protein Interactions
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Pathophysiological Analysis of Nuclear Receptor Function
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Back Matter
Keywords
About this book
Acting principally to control patterns of gene expression, nuclear receptors play vital roles during embryonic development and in the regulation of metabolic and reproductive functions in adult life, which proves this superfamily of ligand-activated transcription factors to be a crucial part of biological life. In The Nuclear Receptor Superfamily: Methods and Protocols, expert researchers describe a range of molecular, structural and cell biological techniques currently used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new therapeutic strategies for treating nuclear receptor-associated diseases. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as notes from the experts to highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, The Nuclear Receptor Superfamily: Methods and Protocols provides beneficial and time-saving guidance for all those undertaking research in this ever-growing field of study.
Reviews
From the reviews:
"The primary focus is on cellular biology, biochemistry and molecular biology. … It is written for a wide range of basic science and clinical researchers interested in transcriptional control of gene expression … . Biomedical scientists and clinicians … will appreciate the wealth of new information presented by the authors, all experts in the field. … This is an outstanding contribution to the literature. … This carefully edited collection of protocols will be valued by all those interested in steroid hormones and nuclear receptors." (Bruce A. Fenderson, Doody’s Review Service, May, 2009)
“This book form part of the series Methods in Molecular Biology and describes methods commonly used to investigate the structural and functional relationship of nuclear receptors, as well as the physiopathology and potential pharmacologic agents for use in therapy of these disorders. … On consulting this book, researchers, students and professionals in the areas of biology and public health will have an opportunity to update their knowledge of experimental methods and applications of functional studies of nuclear receptors and their therapeutic implications.” (Rosario D. C. Hirata, Brazilian Journal of Pharmaceutical Science, Spring, 2010)Editors and Affiliations
Bibliographic Information
Book Title: The Nuclear Receptor Superfamily
Book Subtitle: Methods and Protocols
Editors: Iain J. McEwan
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-575-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60327-574-3Published: 12 December 2008
Softcover ISBN: 978-1-61737-913-0Published: 19 November 2010
eBook ISBN: 978-1-60327-575-0Published: 01 January 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: 240
Number of Illustrations: 39 b/w illustrations, 4 illustrations in colour
Topics: Cell Biology, Gene Expression