Methods in Molecular Biology

The Nuclear Receptor Superfamily

Methods and Protocols

Editors: McEwan, PhD, Iain J. (Ed.)

  • Provides an easily accessible reference for a range of biochemical and cell-based methods to study nuclear receptor function
  • Clear step-by-step comprehensive guides written by experienced researchers in the field
  • Covers cutting-edge methodologies, including ChIP-on-chip, siRNA gene silencing and tissues selective gene knock-outs
  • Details analysis from isolated receptor proteins through to cellular and tissue function
  • Presents experimental approaches, including the use of model biological systems and high-throughput screening, that can be applied to translational research
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-60327-575-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-60327-574-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-61737-913-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Acting principally to control patterns of gene expression, nuclear receptors play vital roles during embryonic development and in the regulation of metabolic and reproductive functions in adult life, which proves this superfamily of ligand-activated transcription factors to be a crucial part of biological life.  In The Nuclear Receptor Superfamily: Methods and Protocols, expert researchers describe a range of molecular, structural and cell biological techniques currently used to investigate the structure-function of nuclear receptors, together with experimental approaches that may lead to new therapeutic strategies for treating nuclear receptor-associated diseases.  Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume contain brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as notes from the experts to highlight tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, The Nuclear Receptor Superfamily: Methods and Protocols provides beneficial and time-saving guidance for all those undertaking research in this ever-growing field of study.

Reviews

From the reviews:

"The primary focus is on cellular biology, biochemistry and molecular biology. … It is written for a wide range of basic science and clinical researchers interested in transcriptional control of gene expression … . Biomedical scientists and clinicians … will appreciate the wealth of new information presented by the authors, all experts in the field. … This is an outstanding contribution to the literature. … This carefully edited collection of protocols will be valued by all those interested in steroid hormones and nuclear receptors." (Bruce A. Fenderson, Doody’s Review Service, May, 2009)

“This book form part of the series Methods in Molecular Biology and describes methods commonly used to investigate the structural and functional relationship of nuclear receptors, as well as the physiopathology and potential pharmacologic agents for use in therapy of these disorders. … On consulting this book, researchers, students and professionals in the areas of biology and public health will have an opportunity to update their knowledge of experimental methods and applications of functional studies of nuclear receptors and their therapeutic implications.” (Rosario D. C. Hirata, Brazilian Journal of Pharmaceutical Science, Spring, 2010)

Table of contents (15 chapters)

  • Nuclear Receptors: One Big Family

    McEwan, Iain J.

    Pages 3-18

  • Methods for Measuring Ligand Dissociation and Nuclear Receptor Turnover in Whole Cells

    Wilson, Elizabeth M.

    Pages 21-33

  • Flow Cytometry as a Tool for Measurement of Steroid Hormone Receptor Protein Expression in Leukocytes

    Butts, Cherie L. (et al.)

    Pages 35-50

  • X-Ray Crystallography of Agonist/Antagonist-Bound Receptors

    Pike, Ashley C.W.

    Pages 51-66

  • FRAP and FRET Methods to Study Nuclear Receptors in Living Cells

    Royen, Martin E. (et al.)

    Pages 69-96

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-60327-575-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-60327-574-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-61737-913-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
The Nuclear Receptor Superfamily
Book Subtitle
Methods and Protocols
Editors
  • Iain J. McEwan, PhD
Series Title
Methods in Molecular Biology
Series Volume
505
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-575-0
DOI
10.1007/978-1-60327-575-0
Hardcover ISBN
978-1-60327-574-3
Softcover ISBN
978-1-61737-913-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
240
Number of Illustrations and Tables
39 b/w illustrations, 4 illustrations in colour
Topics