Methods in Molecular Biology

DNA Damage Detection In Situ, Ex Vivo, and In Vivo

Methods and Protocols

Editors: Didenko, Vladimir V. (Ed.)

  • • The first publication of its kind combines in situ, ex vivo and in vivo techniques
  • • Comprehensive evaluation of various types of DNA damage
  • • Detection in live tissues, blood, urine, sperm, cell cultures and tissue sections
  • • Detection range from subcellular to the level of a whole live organism
  • • Methods for noninvasive detection and monitoring of DNA damage
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-60327-409-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-60327-408-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism. Chapters present all major assays used in molecular and cellular biology for the labeling of DNA damage in situ, ex vivo, and in vivo. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science.

Reviews

From the reviews:

“This volume is presenting the major essays used nowadays in cell and molecular biology laboratories to detect in situ, ex vivo and in vivo DNA damages. Enjoyable and interesting enough is the long explanation devoted by Prof. Didenko … to the use of the terms in vivo, ex vivo and in situ … . Well done! … . Those of us interested in whatsoever way to DNA damage studies will surely find this book very useful.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

Table of contents (22 chapters)

  • In Situ Detection of Apoptosis by the TUNEL Assay: An Overview of Techniques

    Loo, Deryk T.

    Pages 3-13

  • Combination of TUNEL Assay with Immunohistochemistry for Simultaneous Detection of DNA Fragmentation and Oxidative Cell Damage

    Kalyuzhny, Alexander E.

    Pages 15-27

  • EM-ISEL: A Useful Tool to Visualize DNA Damage at the Ultrastructural Level

    Migheli, Antonio

    Pages 29-36

  • In Situ Labeling of DNA Breaks and Apoptosis by T7 DNA Polymerase

    Didenko, Vladimir V.

    Pages 37-48

  • In Situ Ligation: A Decade and a Half of Experience

    Hornsby, Peter J. (et al.)

    Pages 49-63

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-60327-409-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-60327-408-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
DNA Damage Detection In Situ, Ex Vivo, and In Vivo
Book Subtitle
Methods and Protocols
Editors
  • Vladimir V. Didenko
Series Title
Methods in Molecular Biology
Series Volume
682
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60327-409-8
DOI
10.1007/978-1-60327-409-8
Hardcover ISBN
978-1-60327-408-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 313
Topics