Methods in Molecular Biology

Lipoproteins and Cardiovascular Disease

Methods and Protocols

Editors: Freeman, Lita A. (Ed.)

  • Encompasses lipoprotein-centered methodologies designed for all experience levels
  • Features detailed protocols that are lab-ready
  • Includes expert tips and key implementation advice to ensure successful results
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-60327-369-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-60327-368-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-5690-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The lipid-rich and otherwise challenging nature of many key tissues complicates many aspects of current research, and applications of the unique nature of lipoproteins and their biological effects has engendered unique and vital methodologies.  In Lipoproteins and Cardiovascular Disease: Methods and Protocols, experts in the field present a compendium of advanced and classical molecular biology methods targeted towards lipoprotein, atherosclerosis, and vascular biology research, bringing together in a single volume an updated set of protocols and strategies for methods now driving the most recent advances, along with classical methods that are still widely used.  Among the many topics covered in this cutting-edge work, the book delves into crucial techniques such as quantitative real-time PCR, microarrays, RT-PCR laser capture microdissection, and tissue-specific gene overexpression, knockout, and knockdown methodologies, including AAV as a liver-directed gene delivery vehicle.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable notes which highlight tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and easy to use, Lipoproteins and Cardiovascular Disease: Methods and Protocols serves both novices and experts alike as a complete guide for any researcher with an interest in lipoproteins and their significant biological effects.

Table of contents (18 chapters)

  • Cloning Full-Length Transcripts and Transcript Variants Using 5′ and 3′ RACE

    Freeman, Lita A.

    Pages 3-17

  • Monitoring Gene Expression: Quantitative Real-Time RT-PCR

    Wagner, Elke M.

    Pages 19-45

  • Microarray Technology: Basic Methodology and Application in Clinical Research for Biomarker Discovery in Vascular Diseases

    Raghavachari, Nalini

    Pages 47-84

  • Northern Analysis of Gene Expression

    Freeman, Lita A.

    Pages 85-121

  • Laser Capture Microdissection for Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

    Feig, Jonathan E. (et al.)

    Pages 123-135

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-60327-369-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-60327-368-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-4939-5690-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Lipoproteins and Cardiovascular Disease
Book Subtitle
Methods and Protocols
Editors
  • Lita A. Freeman
Series Title
Methods in Molecular Biology
Series Volume
1027
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60327-369-5
DOI
10.1007/978-1-60327-369-5
Hardcover ISBN
978-1-60327-368-8
Softcover ISBN
978-1-4939-5690-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 391
Number of Illustrations and Tables
27 b/w illustrations, 23 illustrations in colour
Topics