Methods in Molecular Biology

Helicases

Methods and Protocols

Editors: Abdelhaleem, Mohamed M. (Ed.)

  • Provides full coverage of helicases, the enzymes that unwind the double stranded helical structure of nucleic acids
  • Written by leading international experts in the field
  • Includes a wide range of methods in different species
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-60327-355-8
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Hardcover $169.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-60327-354-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-61779-667-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

In recent years, much information has been revealed concerning the essential role of helicases, the enzymes that utilize the energy derived from nucleoside triphosphate hydrolysis to unwind the double stranded helical structure of nucleic acids. In Helicases: Methods and Protocols, this topic is explored by leading international scientists who have contributed significantly to our current understanding of helicases and the ways in which they function. Chapters include methods for the production and purification of helicases from different species, as well as detailed studies of helicase activities, including NTP binding and hydrolysis, nucleic acid binding and unwinding, and translocation along nucleic acid substrates. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Wide-ranging and innovative, Helicases: Methods and Protocols is the ultimate guide for researchers, providing the scientific community with comprehensive accounts of the most advanced methods used to study helicases today.

Table of contents (27 chapters)

  • Helicases: An Overview

    Abdelhaleem, Mohamed

    Pages 1-12

  • Fluorescent Biosensors to Investigate Helicase Activity

    Webb, Martin R.

    Pages 13-27

  • Single-Molecule FRET Analysis of Helicase Functions

    Rothenberg, Eli (et al.)

    Pages 29-43

  • Kinetics of Motor Protein Translocation on Single-Stranded DNA

    Fischer, Christopher J. (et al.)

    Pages 45-56

  • Experimental and Computational Analysis of DNA Unwinding and Polymerization Kinetics

    Pandey, Manjula (et al.)

    Pages 57-83

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-60327-355-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-60327-354-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-61779-667-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Helicases
Book Subtitle
Methods and Protocols
Editors
  • Mohamed M. Abdelhaleem
Series Title
Methods in Molecular Biology
Series Volume
587
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-355-8
DOI
10.1007/978-1-60327-355-8
Hardcover ISBN
978-1-60327-354-1
Softcover ISBN
978-1-61779-667-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 404
Topics