Methods in Molecular Biology

PCR Detection of Microbial Pathogens

Editors: Wilks, Mark (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-60327-353-4
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Hardcover $139.00
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About this book

PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one’s own tests or introducing tests developed by other workers. PCR Detection of Microbial Pathogens, Second Edition presents alternatives to commercially produced PCR methods to detect microbial pathogens. Although most of the chapters in this book are devoted to the detection of specific pathogens, the first  chapters in this book should appeal to anyone working in this field regardless of their particular interests. Although PCR tests can often be made to work with relatively little effort, it is often unclear how efficient the PCR test is, how inhibitory the specimen containing the pathogen of interest is and how the test can be quality controlled. All of which are of great importance in developing tests for diagnostic use. These topics are covered  in great depth at the beginning of the book. The main part of the book is devoted to describing methods for the detection of a wide range of pathogens and from widely different specimens and situations. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and accessible, PCR Detection of Microbial Pathogens, Second Edition serves microbiologists regardless of their particular interest because, when used together with the general principles, the sheer variety of procedures provided here enables the reader to design and introduce diagnostic tests in the laboratory with confidence.

Table of contents (21 chapters)

  • Real-Time Quantitative PCR, Pathogen Detection and MIQE

    Johnson, Gemma (et al.)

    Pages 1-16

  • Overcoming Inhibition in Real-Time Diagnostic PCR

    Hedman, Johannes (et al.)

    Pages 17-48

  • Quality in the Molecular Microbiology Laboratory

    Wallace, Paul S. (et al.)

    Pages 49-79

  • Pre-analytical Sample Treatment and DNA Extraction Protocols for the Detection of Bacterial Pathogens from Whole Blood

    Hansen, Wendy L. J. (et al.)

    Pages 81-90

  • Detection of Bacterial Contamination in Platelet Concentrates Using Flow Cytometry and Real-Time PCR Methods

    Vollmer, Tanja (et al.)

    Pages 91-103

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-60327-353-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-60327-352-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
PCR Detection of Microbial Pathogens
Editors
  • Mark Wilks
Series Title
Methods in Molecular Biology
Series Volume
943
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60327-353-4
DOI
10.1007/978-1-60327-353-4
Hardcover ISBN
978-1-60327-352-7
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XI, 317
Topics