Methods in Molecular Biology

Retinoids

Methods and Protocols

Editors: Sun, Hui, Travis, Gabriel H. (Eds.)

  • Comprehensive technical guides to the roles of vitamin A and retinoids in diverse physiological and pathological states and molecular pathways
  • Up-to-date descriptions of biochemical, biophysical, and cell-biological techniques
  • Easily accessible reference volume for beginners and experts
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-60327-325-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-60327-324-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
  • ISBN 978-1-61779-690-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids. In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies. Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and quantitation of retinoids using HPLC, mass spectrometry, and fluorescence, fluorescence anisotropy of retinol binding protein, cell culture models for studying retinoid transport and the role of retinol in embryonic stem cell culture, as well as many other detailed procedures. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search to uncover even more vital aspects of vitamin A’s impact on the human body.

Reviews

From the reviews:

“This excellent book provides authoritative and cutting-edge information in the field of retinoids … . It is intended both for beginning and experienced researchers in diverse fields who are interested in learning new techniques. Even graduate students, nutritionists, and clinicians could find this book informative and helpful. … By combining biochemical, biophysical, and cell biological techniques, the book thoroughly addresses the field of retinoid research.” (Omer Iqbal, Doody’s Review Service, October, 2010)

Table of contents (20 chapters)

  • Quantification of Endogenous Retinoids

    Kane, Maureen A. (et al.)

    Pages 1-54

  • Culture of Highly Differentiated Human Retinal Pigment Epithelium for Analysis of the Polarized Uptake, Processing, and Secretion of Retinoids

    Hu, Jane (et al.)

    Pages 55-73

  • Feeder-Independent Culture of Mouse Embryonic Stem Cells Using Vitamin A/Retinol

    Khillan, Jaspal S. (et al.)

    Pages 75-83

  • In Vitro Assays of Rod and Cone Opsin Activity: Retinoid Analogs as Agonists and Inverse Agonists

    Kono, Masahiro (et al.)

    Pages 85-94

  • Physiological Studies of the Interaction Between Opsin and Chromophore in Rod and Cone Visual Pigments

    Kefalov, Vladimir J. (et al.)

    Pages 95-114

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-60327-325-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-60327-324-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
  • ISBN 978-1-61779-690-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Retinoids
Book Subtitle
Methods and Protocols
Editors
  • Hui Sun
  • Gabriel H. Travis
Series Title
Methods in Molecular Biology
Series Volume
652
Copyright
2010
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60327-325-1
DOI
10.1007/978-1-60327-325-1
Hardcover ISBN
978-1-60327-324-4
Softcover ISBN
978-1-61779-690-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 350
Topics