Methods in Molecular Biology

Protein Folding, Misfolding, and Disease

Methods and Protocols

Editors: Hill, A.F., Barnham, K.J., Bottomley, S.P., Cappai, R. (Eds.)

  • Brings together a thorough collection of cutting-edge methods for protein folding and misfolding
  • Features concise, step-by-step approaches to the varying topics
  • Includes key tips and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-60327-223-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-60327-221-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Protein misfolding is a key feature of many disorders in humans, given that over twenty proteins are known to misfold and cause disease.  In Protein Folding, Misfolding, and Disease: Methods and Protocols, experts in the field present a collection of current methods for studying the analysis of protein folding and misfolding, featuring strategies for expressing and refolding recombinant proteins which can then be utilized in subsequent experiments. This detailed volume also covers methods for analyzing the formation of amyloid, protocols for determining the size and structure of native and misfolded proteins, as well as specific examples of where misfolded proteins can be examined using state-of –the-art technologies. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Up to date and authoritative, Protein Folding, Misfolding, and Disease: Methods and Protocols offers researchers the tools necessary to move ahead in this vital field.

Table of contents (15 chapters)

  • Strategies for Boosting the Accumulation of Correctly Folded Recombinant Proteins Expressed in Escherichia coli

    Marco, Ario

    Pages 1-15

  • An Escherichia coli Cell-Free System for Recombinant Protein Synthesis on a Milligram Scale

    Miles, Luke A. (et al.)

    Pages 17-28

  • Synthesis of Peptide Sequences Derived from Fibril-Forming Proteins

    Scanlon, Denis B. (et al.)

    Pages 29-43

  • Refolding Your Protein with a Little Help from REFOLD

    Phan, Jennifer (et al.)

    Pages 45-57

  • Circular Dichroism and Its Use in Protein-Folding Studies

    Clarke, David T.

    Pages 59-72

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-60327-223-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-60327-221-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein Folding, Misfolding, and Disease
Book Subtitle
Methods and Protocols
Editors
  • Andrew F. Hill
  • Kevin J. Barnham
  • Stephen P. Bottomley
  • Roberto Cappai
Series Title
Methods in Molecular Biology
Series Volume
752
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60327-223-0
DOI
10.1007/978-1-60327-223-0
Hardcover ISBN
978-1-60327-221-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 249
Topics