Methods in Molecular Biology

Germline Stem Cells

Editors: Hou, Steven X., Singh, Shree Ram (Eds.)

  • Readily reproducible protocols for the manipulation of germline stem cells in model organisms
  • State-of-the-art methods optimized by leading experts in germline stem cells
  • Identification and regulation of germline stem cell in model organisms
  • Current techniques used in in-vitro culture and application in germline stem cells
  • Presents sophisticated genetic, molecular and cellular techniques used in germline stem cell research
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-60327-214-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.00
price for USA
  • ISBN 978-1-60327-213-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA
  • ISBN 978-1-61737-880-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The knowledge gained by studying germline stem cells, the source of human and animal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors.  In Germline Stem Cells, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them.  Divided into two parts, the volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells.  Following the highly successful Methods in Molecular Biology™ series format, each chapter includes a brief introduction to the subject, a list of necessary equipment and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Germline Stem Cells is the perfect reference for scientists exploring this particular exciting and important subdivision in the fast-paced field of stem cell research.

Reviews

From the reviews:

"This book provides background information and protocols for identifying, isolating, characterizing, and manipulating … germline stem cells. … This book is written for basic science and clinical researchers … as well as those interested in development and reproductive biology. Biomedical scientists and clinicians … will also appreciate the wealth of new information. … This is an outstanding contribution to the literature in germ cell biology. ... will be valued by all those interested in development of the germ lineage and stem cell biology." (Bruce A. Fenderson, Doody’s Review Service, October, 2008)


Table of contents (16 chapters)

  • The Development of Germline Stem Cells in Drosophila

    Dansereau, David A. (et al.)

    Pages 3-26

  • Analysis of the C. elegans Germline Stem Cell Region

    Crittenden, Sarah L. (et al.)

    Pages 27-44

  • Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell

    Singh, Shree Ram (et al.)

    Pages 45-59

  • Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells

    Liu, Wei (et al.)

    Pages 61-70

  • Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (Milkweed Bug Oncopeltus fasciatus)

    Dorn, David C. (et al.)

    Pages 71-94

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-60327-214-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.00
price for USA
  • ISBN 978-1-60327-213-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA
  • ISBN 978-1-61737-880-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Germline Stem Cells
Editors
  • Steven X. Hou
  • Shree Ram Singh
Series Title
Methods in Molecular Biology
Series Volume
450
Copyright
2008
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-214-8
DOI
10.1007/978-1-60327-214-8
Hardcover ISBN
978-1-60327-213-1
Softcover ISBN
978-1-61737-880-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 273
Topics