Methods in Molecular Biology

2D PAGE: Sample Preparation and Fractionation

Volume 2

Editors: Posch, Anton (Ed.)

  • Sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics
  • Shows how to perform two-dimensional electrophoresis with step-by-step instructions
  • Ensures successful results with tricks of the trade and troubleshooting tips
  • Offers methods that can be applied to a broad range of protein separation techniques
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-60327-210-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-60327-209-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
  • ISBN 978-1-61737-879-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 2 illuminates specific applications of sample preparation tools and fractionation strategies to study a variety of biological systems.

 

Invaluable for students of Biochemistry, Biomedicine, Biology and Genomics and essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delves into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field.

Table of contents (25 chapters)

  • Application of Fluorescence Dye Saturation Labeling for Differential Proteome Analysis of 1,000 Microdissected Cells from Pancreatic Ductal Adenocarcinoma Precursor Lesions

    Sitek, Barbara (et al.)

    Pages 1-14

  • Albumin and Immunoglobulin Depletion of Human Plasma

    Jenkins, Rosalind E. (et al.)

    Pages 15-25

  • Multi-Component Immunoaffinity Subtraction and Reversed-Phase Chromatography of Human Serum

    Martosella, James (et al.)

    Pages 27-39

  • Immunoaffinity Fractionation of Plasma Proteins by Chicken IgY Antibodies

    Huang, Lei (et al.)

    Pages 41-51

  • Proteomics of Cerebrospinal Fluid: Methods for Sample Processing

    Hale, John E. (et al.)

    Pages 53-66

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-60327-210-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-60327-209-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
  • ISBN 978-1-61737-879-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
2D PAGE: Sample Preparation and Fractionation
Book Subtitle
Volume 2
Editors
  • Anton Posch
Series Title
Methods in Molecular Biology
Series Volume
425
Copyright
2008
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-210-0
DOI
10.1007/978-1-60327-210-0
Hardcover ISBN
978-1-60327-209-4
Softcover ISBN
978-1-61737-879-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 340
Number of Illustrations and Tables
80 b/w illustrations, 3 illustrations in colour
Topics