2D PAGE: Sample Preparation and Fractionation

1. Front Matter

   Pages i-xiii

   PDF

2. Application of Fluorescence Dye Saturation Labeling for Differential Proteome Analysis of 1,000 Microdissected Cells from Pancreatic Ductal Adenocarcinoma Precursor Lesions

   • Barbara Sitek, Bence Sipos, Günter Klöppel, Wolff Schmiegel, Stephan A Hahn, Helmut E Meyer et al.

   Pages 1-14

3. Albumin and Immunoglobulin Depletion of Human Plasma

   • Rosalind E. Jenkins, Neil R. Kitteringham, Carrie Greenough, B. Kevin Park

   Pages 15-25

4. Multi-Component Immunoaffinity Subtraction and Reversed-Phase Chromatography of Human Serum

   • James Martosella, Nina Zolotarjova

   Pages 27-39

5. Immunoaffinity Fractionation of Plasma Proteins by Chicken IgY Antibodies

   • Lei Huang, Xiangming Fang

   Pages 41-51

6. Proteomics of Cerebrospinal Fluid: Methods for Sample Processing

   • John E. Hale, Valentina Gelfanova, Jin-Sam You, Michael D. Knierman, Robert A. Dean

   Pages 53-66

7. Sample Preparation of Bronchoalveolar Lavage Fluid

   • Baptiste Leroy, Paul Falmagne, Ruddy Wattiez

   Pages 67-75

8. Preparation of Nasal Secretions for Proteome Analysis

   • Begona Casado, Paolo Iadarola, Lewis K Pannell

   Pages 77-87

9. Preparation of Urine Samples for Proteomic Analysis

   • Rembert Pieper

   Pages 89-99

10. Isolation of Cytoplasmatic Proteins from Cultured Cells for Two-Dimensional Gel Electrophoresis

    • Ying Wang, Jen-Fu Chiu, Qing-Yu He

    Pages 101-112

11. Sample Preparation of Culture Medium from Madin-Darby Canine Kidney Cells

    • Daniel Ambort, Daniel Lottaz, Erwin Sterchi

    Pages 113-130

12. Sample Preparation for Mass Spectrometry Analysis of Formalin-Fixed Paraffin-Embedded Tissue

    • Nicolas A Stewart, Timothy D Veenstra

    Pages 131-138

13. Metalloproteomics in the Molecular Study of Cell Physiology and Disease

    • Hermann-Josef Thierse, Stefanie Helm, Patrick Pankert

    Pages 139-147

14. Protein Extraction from Green Plant Tissue

    • Ragnar Flengsrud

    Pages 149-152

15. The Terminator: A Device for High-Throughput Extraction of Plant Material

    • B.M. van den Berg

    Pages 153-162

16. Isolation of Mitochondria from Plant Cell Culture

    • Etienne H. Meyer, A. Harvey Millar

    Pages 163-169

17. Isolation and Preparation of Chloroplasts from Arabidopsis thaliana Plants

    • Sybille E Kubis, Kathryn S Lilley, Paul Jarvis

    Pages 171-186

18. Isolation of Plant Cell Wall Proteins

    • Elisabeth Jamet, Georges Boudart, Giséle Borderies, Stephane Charmont, Claude Lafitte, Michel Rossignol et al.

    Pages 187-201

19. Isolation and Fractionation of the Endoplasmic Reticulum from Castor Bean (Ricinus communis) Endosperm for Proteomic Analyses

    • William J Simon, Daniel J Maltman, Antoni R Slabas

    Pages 203-215

20. Cell Wall Fractionation for Yeast and Fungal Proteomics

    • Aida Pitarch, César Nombela, Concha Gil

    Pages 217-239

The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 2 illuminates specific applications of sample preparation tools and fractionation strategies to study a variety of biological systems.

 

Invaluable for students of Biochemistry, Biomedicine, Biology and Genomics and essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delves into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field.

• 2-DE
• Arabidopsis thaliana
• Chloroplast
• Cytoplasma
• Electrophoresis
• Expression Proteomics
• Polyacrylamid-Gelelektrophorese
• Proteins
• Proteome Analysis
• Proteomics
• Protozoa
• biochemistry
• fungi
• membrane proteins

• Bio-Rad Laboratories GmbH, Germany

  Anton Posch

Policies and ethics