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Methods in Molecular Biology

Protein Secretion

Methods and Protocols

Editors: Economou, Anastassios (Ed.)

  • -Comprehensive guide to developing, running, and analyzing assays to monitor protein secretion from single proteins to proteome scale
  • -Presents a modular approach to experimental design, covering model biological systems, screening modalities, detection technologies, and bioinformatics tools
  • -Covers a wide range of cell-based and in vitro assay formats and systems
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eBook $149.00
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  • ISBN 978-1-60327-412-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
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  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-60327-167-7
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About this book

Over the past thirty years, many elegant genetic and biochemical approaches have been combined in order to advance the study of protein secretion and the necessary navigation through cell membranes, yet, despite this progress, less than two hundred membrane protein structures are known, nowhere near the complete inventory that the discovered protein export systems suggest. In Protein Secretion: Methods and Protocols, leading experts in the field provide robust, well-established protocols to elucidate the multiplicity of tools that have been developed to study protein sorting, membrane targeting, transmembrane crossing, and secretion across multiple membranes. With examples involving both prokaryotic and eukaryotic organisms, the volume covers subjects ranging from bioinformatics and proteomics to fundamental enzymology and genetics to cell biology, structural analyses, and biophysics. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the key materials and reagents, step-by-step, readily reproducible protocols, and detailed notes on troubleshooting and avoiding known pitfalls.

Comprehensive and dependable, Protein Secretion: Methods and Protocols focuses on well-characterized paradigms so that scientists studying a vast array of subjects from biochemistry and genetics to biotechnology and biopharmaceuticals can benefit and expand upon their vital research.

Table of contents (26 chapters)

  • The Extraordinary Diversity of Bacterial Protein Secretion Mechanisms

    Holland, I. Barry

    Pages 1-20

  • In Vitro and In Vivo Approaches to Studying the Bacterial Signal Peptide Processing

    Wang, Peng (et al.)

    Pages 21-37

  • Membrane Insertion of Small Proteins

    Kuhn, Andreas (et al.)

    Pages 39-62

  • Membrane Protein Insertion in E. coli

    Yuan, Jijun (et al.)

    Pages 63-77

  • Study of Polytopic Membrane Protein Topological Organization as a Function of Membrane Lipid Composition

    Bogdanov, Mikhail (et al.)

    Pages 79-101

Buy this book

eBook $149.00
price for USA
  • ISBN 978-1-60327-412-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-60327-167-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein Secretion
Book Subtitle
Methods and Protocols
Editors
  • Anastassios Economou
Series Title
Methods in Molecular Biology
Series Volume
619
Copyright
2010
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-60327-412-8
DOI
10.1007/978-1-60327-412-8
Hardcover ISBN
978-1-60327-167-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 426
Topics