Methods in Molecular Biology

Plant MicroRNAs

Methods and Protocols

Editors: Meyers, Blake C., Green, Pamela J. (Eds.)

  • Presents the fundamentals of plant miRNA biology, including pathways and approaches used for their discovery and investigation, as written by leading researchers in the field
  • Covers a broad range of molecular approaches to the study of small RNA, from basic to advanced experimental and computational techniques
  • Details the methods by which the expression of miRNAs and their impact on mRNA levels are characterized, including deep sequencing methods for miRNAs and target RNAs
  • Enables molecular biologists to design and perform experiments on the biology of plant miRNAs and small RNAs, including their detection, quantification and localization, and covers approaches to data analysis
  • Includes applications and advanced adaptations of techniques relevant to all plant biologists
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-60327-005-2
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  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-60327-004-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-61779-653-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects.  In Plant MicroRNAs: Methods and Protocols, experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs.  In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another.  As a volume in the highly successful Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easy to use, Plant MicroRNAs: Methods and Protocols provides the research community with a set of protocols that will help advance vital miRNA research for all plant species, both in typical model species and non-model species alike.

Table of contents (17 chapters)

  • Piecing the Puzzle Together: Genetic Requirements for miRNA Biogenesis in Arabidopsis thaliana

    Xie, Zhixin

    Pages 1-17

  • Prediction of Plant miRNA Genes

    Jones-Rhoades, Matthew W.

    Pages 19-30

  • Methods for Isolation of Total RNA to Recover miRNAs and Other Small RNAs from Diverse Species

    Accerbi, Monica (et al.)

    Pages 31-50

  • miRNA Target Prediction in Plants

    Fahlgren, Noah (et al.)

    Pages 51-57

  • A Method to Discover Phased siRNA Loci

    Axtell, Michael J.

    Pages 59-70

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-60327-005-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-60327-004-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-61779-653-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Plant MicroRNAs
Book Subtitle
Methods and Protocols
Editors
  • Blake C. Meyers
  • Pamela J. Green
Series Title
Methods in Molecular Biology
Series Volume
592
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-005-2
DOI
10.1007/978-1-60327-005-2
Hardcover ISBN
978-1-60327-004-5
Softcover ISBN
978-1-61779-653-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 261
Number of Illustrations and Tables
46 b/w illustrations
Topics