Methods in Molecular Biology

Apoptosis Methods and Protocols

Editors: Brady, Hugh J. M. (Ed.)

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About this book

The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research in the 21st century. In Apoptosis Methods and Protocols, highly experienced researchers in the field of apoptosis describe in step-by-step detail the techniques they have perfected to investigate the critical steps involved in the apoptotic process. These readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Apoptosis Methods and Protocols constitutes a key technical reference to the significant methodologies used in the field, as well as offering novice and experienced researchers alike powerful tools to illuminate the phenomenon of programmed cell death.

Reviews

"The calibre of the contributors to this book, make it an authoritative tome. As it also provides insights in to how and why specific protocols should be applied, it will be a great asset to those studying apoptosis in laboratory-based haematology." -British Journal of Haematology

"...well documented...clearly laid out...a daily reference to the laboratory for post graduate students." - Veterinary Pathology

Table of contents (19 chapters)

  • Measurement of Apoptosis by DNA Fragmentation

    Matassov, Demetrius (et al.)

    Pages 1-17

  • Measurement of Caspase Activity in Cells Undergoing Apoptosis

    Kumar, Sharad

    Pages 19-30

  • Flow Cytometry-Based Methods for Apoptosis Detection in Lymphoid Cells

    Williams, Owen

    Pages 31-42

  • Methods of Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays

    Duke, Richard C.

    Pages 43-66

  • Detecting and Quantifying Apoptosis in Tissue Sections

    Save, Vicki (et al.)

    Pages 67-84

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-59259-812-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Apoptosis Methods and Protocols
Editors
  • Hugh J. M. Brady
Series Title
Methods in Molecular Biology
Series Volume
282
Copyright
2004
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-59259-812-0
DOI
10.1385/1592598129
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 304
Number of Illustrations and Tables
74 b/w illustrations
Topics