Molecular Biomethods Handbook

Editors: Rapley, Ralph, Walker, John M. (Eds.)

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  • ISBN 978-1-59259-642-3
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About this book

In the Molecular Biomethods Handbook, Ralph Rapley and John Walker assemble an authoritative team of investigators to illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced writers fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included.

The Molecular Biomethods Handbook provides both established scientists and novices who are new to these techniques a deeper understanding of the widest variety of biotechniques-and how to use them successfully. The resulting insights will significantly enhance your ability to investigate and define biological processes at the molecular level.

Reviews

"A good book for those who have been 'brought up' on kits and for those who would like to understand a little bit more about how a technique works rather than simply use it."-Microbiology Today

". . .a good lab investment."-Trends in Genetics

". . .a good teaching resource for undergraduates learning modern molecular genetic techniques. . .provides a clear introduction to many molecular biology techniques. . .best suited to mouse and human genetics."-Molecular Biotechnology

Table of contents (51 chapters)

  • Extraction of Total RNA from Tissues and Cultured Cells

    Raha, Sandeep (et al.)

    Pages 1-8

  • Isolation of Total Cellular DNA from Tissues and Cultured Cells

    Merante, Frank (et al.)

    Pages 9-16

  • Gel Electrophoresis of DNA

    Smith, Duncan R.

    Pages 17-33

  • Sl Nuclease Analysis of RNA

    Lefebvre, Louis (et al.)

    Pages 35-49

  • Detecting mRNA by Use of the Ribonuclease Protection Assay (RPA)

    Einspanier, Ralf (et al.)

    Pages 51-57

Buy this book

eBook $89.00
price for USA (gross)
  • ISBN 978-1-59259-642-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
Molecular Biomethods Handbook
Editors
  • Ralph Rapley
  • John M. Walker
Copyright
1998
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-59259-642-3
DOI
10.1007/978-1-59259-642-3
Edition Number
1
Number of Pages
XIII, 725
Number of Illustrations and Tables
360 b/w illustrations
Topics