The Protein Protocols Handbook

Editors: Walker, John M. (Ed.)

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  • ISBN 978-1-59259-169-5
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About this book

From Reviews of the First Edition
"...a must for researchers in protein chemistry and especially those with less experience..."
-Doody's Health Sciences Book Review Journal
"...definitely an indispensable benchtop handbook...highly recommended for all levels of research
within the field."-Bioseparation
"...a very useful tool for daily work." -Nahrung-Food

Since publication of the bestselling first edition of John Walker's widely acclaimed Protein Protocols Handbook in 1996, there have been many highly significant developments in protein biochemistry. This greatly enhanced second edition introduces 60 critically important new chapters dealing with topics and techniques that have emerged since the first edition, as well as significantly updating the remaining articles. The new chapters cover many rapidly developing areas, particularly the application of mass spectrometry in protein characterization, as well as the now well-established 2-D PAGE technique in proteomics. The section on glycoprotein analysis has also been significantly expanded, and methods for the production of single-chain and phage-displayed antibodies have been added to the section on antibodies.
Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The authors are commonly the techniques' originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity.
Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Second Edition is today's indispensable benchtop manual and guide-not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armametarium of techniques in the urgent search for rapid and still more robust results.

Reviews

"the breadth of techniques covered is large and this alone makes the book of practical value. . .The protocols are provided with sufficient detail so as to allow implementation as new methods in any working laboratory. . .It can easily be envisioned that this book would become the subject of a tug of war in a busy laboratory. Most investigators will find value here as will students working in almost any area dealing with proteins. . .This is a compilation not found in other single sources. The very broad scope has considerable practical value. I personally want this in my laboratory."-Doody's Health Sciences Book Review Journal

"The wide acceptance of this handbook in the former edition has resulted to this second thoroughly revised and extended second edition considering the recent significant developments in the field of protein chemistry. It is an excellent collection of methods and protocols, giving an essential and compact guide for those new as well as those well established to protein laboratory techniques." - Ernahrungforschung

Review of the first edition:
"The Protein Protocols Handbook has arrived at the right place at the right time."-Trends in Cell Biology

From Reviews of the First Edition
"...a must for researchers in protein chemistry and especially those with less experience..."
-Doody's Health Sciences Book Review Journal
"...a very useful tool for daily work.." -Nahrung-Food
"...definitely an indispensable benchtop handbook...highly recommended for all levels of research within the field." -Bioseparation
"...stands out in an already overcrowded field..."
-Aslib Book Guide

"In his preface the editor wishes the reader luck in his protein analysis, his book represents an invaluable compendium to help achieve this aim." - Molecular Biotechnology

Table of contents (164 chapters)

  • Protein Determination by UV Absorption

    Aitken, Alastair (et al.)

    Pages 3-6

  • The Lowry Method for Protein Quantitation

    Waterborg, Jakob H.

    Pages 7-9

  • The Bicinchoninic Acid (BCA) Assay for Protein Quantitation

    Walker, John M.

    Pages 11-14

  • The Bradford Method for Protein Quantitation

    Kruger, Nicholas J.

    Pages 15-21

  • Ultrafast Protein Determinations Using Microwave Enhancement

    Akins, Robert E. (et al.)

    Pages 23-30

Buy this book

eBook $69.99
price for USA (gross)
  • ISBN 978-1-59259-169-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
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Bibliographic Information

Bibliographic Information
Book Title
The Protein Protocols Handbook
Editors
  • John M. Walker
Copyright
2002
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-169-5
DOI
10.1385/1592591698
Edition Number
2
Number of Pages
XXIV, 1146
Topics