Methods in Molecular Biology

The Nucleus

Volume 1: Nuclei and Subnuclear Components

Editors: Hancock, Ronald (Ed.)

  • Includes state-of-the-art procedures for isolating and working with nuclei from a wide range of cell types, and procedures for studying their components including pathology-related structures

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-59745-406-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-58829-977-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-61737-863-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development of the new, powerful biochemical and biophysical methodologies described here are essential in this quest. In The Nucleus, researchers from more than forty leading international laboratories describe state-of-the-art methods for isolating nuclei and their components and for studying their structure and activities, including some pathology-associated features. Volume I: Nuclei and Subnuclear Structures presents an overview of features of the intranuclear environment, followed by the most recent procedures for isolating nuclei from a wide range of cell types including muscle cells, yeast, oocytes, cells with polytene nuclei, Arabidopsis, trypanosomes, and dinoflagellates. The latest methods are described for isolating and working with nucleoli, constitutive heterochromatin, pathology-associated inclusions, and chromatin and for examining glycosylation, sumoylation, and ADP-ribosylation of nuclear proteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain lists of necessary materials and reagents, readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls.

The Nucleus, Volume I: Nuclei and Subnuclear Structures is an essential reference for scientists who are working on our rapidly growing understanding of the cell nucleus and its activities.

Table of contents (19 chapters)

  • The Intranuclear Environment

    Schnell, Santiago (et al.)

    Pages 3-19

  • Purification of Nuclei and Preparation of Nuclear Envelopes from Skeletal Muscle

    Wilkie, Gavin S. (et al.)

    Pages 23-41

  • Isolation of Highly Purified Yeast Nuclei for Nuclease Mapping of Chromatin Structure

    Reese, Joseph C. (et al.)

    Pages 43-53

  • Working with Oocyte Nuclei: Cytological Preparations of Active Chromatin and Nuclear Bodies from Amphibian Germinal Vesicles

    Morgan, Garry T.

    Pages 55-66

  • Preparation of Arabidopsis Nuclei and Nucleoli

    McKeown, Peter (et al.)

    Pages 67-75

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-59745-406-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-58829-977-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-61737-863-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
The Nucleus
Book Subtitle
Volume 1: Nuclei and Subnuclear Components
Editors
  • Ronald Hancock
Series Title
Methods in Molecular Biology
Series Volume
463
Copyright
2008
Publisher
Humana Press
Copyright Holder
Humana Press
Distribution Rights
Distribution rights for India: Atlantic Pub. & Distr. (P) Ltd., New Delhi, India
eBook ISBN
978-1-59745-406-3
DOI
10.1007/978-1-59745-406-3
Hardcover ISBN
978-1-58829-977-2
Softcover ISBN
978-1-61737-863-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVIII, 323
Topics