Methods in Molecular Biology

Functional Proteomics

Methods and Protocols

Editors: Thompson, Julie D., Schaeffer-Reiss, Christine, Ueffing, Marius (Eds.)

  • Unique combination of reproducible laboratory protocols and state-of-the-art bioinformatics methods dedicated to functional proteomics
  • A comprehensive methodological compendium, covering sample preparation, protein separation, mass-spectrometry as well as data mining, documentation and integration
  • Offers methods, workflows and strategies to overcome current pitfalls and shortcomings in proteome analysis
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eBook $159.00
price for USA (gross)
  • ISBN 978-1-59745-398-1
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $209.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-58829-971-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $209.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-61737-860-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

As the emerging field of proteomics continues to expand at an extremely rapid rate, the relative quantification of proteins, targeted by their function, becomes its greatest challenge. Complex analytical strategies have been designed that allow comparative analysis of large proteomes, as well as in depth detection of the core proteome or the interaction network of a given protein of interest. In Functional Proteomics: Methods and Protocols, expert researchers describe the latest protocols being developed to address the problems encountered in high-throughput proteomics projects, with emphasis on the factors governing the technical choices for given applications. The case studies within the volume focus on the following three crucial aspects of the experimental design: 1) the strategy used for the selection, purification and preparation of the sample to be analyzed by mass spectrometry, 2) the type of mass spectrometer used and the type of data to be obtained from it, and 3) the method used for the interpretation of the mass spectrometry data and the search engine used for the identification of the proteins in the different types of sequence data banks available. As a part of the highly successful Methods in Molecular Biology™ series, the chapters compile step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Functional Proteomics: Methods and Protocols is an ideal resource for all scientists pursuing this developing field and its multitudinous data.

Reviews

From the reviews: “The new book, Functional Proteomics, Methods and Protocols … is the perfect guide to securely navigating through the plethora of recent as well as established proteomic technologies. … In summary, I highly recommend this book as an excellent source for gaining insight into a diverse array of the latest proteomic protocols … . suitable for everyone working in the field of proteomics, including chemists, biologists, analytical chemists, and biochemists at different career stages, from students to the trained and experienced scientist.” (Stephan A. Sieber, ChemBioChem, Vol. 10 (18), 2009) “Book presents a collection of methods about correct techniques to identify, monitor, quantify, and explain protein and peptide function. … present different types of mass spectrometers and their uses, allowing the reader to choose the most useful technique for a specific purpose. … The strategy behind metabolomic studies is well described in this book. … The book concludes with the indispensable application of bioinformatics in the understanding of regulatory and protein systems which are influenced by the environment.” (Mario Hiroyuki Hirata, Brazilian Journal of Pharmaceutical Sciences, 2010) “The field of proteomics continues to develop rapidly and its main focus has now moved from increasing capacity for protein identification to quantification and the analysis of post-translational modifications. … this volume attempts to provide detail regarding some of the latest approaches being used. … will be of use to many PhD students and postdoctoral scientists … . a well-written and informative book, and one which would be useful to have in any proteomics lab … .” (Lucas Bowler, Microbiology Today, August, 2010)

Table of contents (33 chapters)

  • A Brief Summary of the Different Types of Mass Spectrometers Used in Proteomics

    Schaeffer-Reiss, Christine

    Pages 3-16

  • Experimental Setups and Considerations to Study Microbial Interactions

    Melin, Petter

    Pages 17-26

  • Plant Proteomics

    Sarnighausen, Eric (et al.)

    Pages 29-44

  • Methods for Human CD8+ T Lymphocyte Proteome Analysis

    Thadikkaran, Lynne (et al.)

    Pages 45-65

  • Label-Free Proteomics of Serum

    Govorukhina, Natalia (et al.)

    Pages 67-77

Buy this book

eBook $159.00
price for USA (gross)
  • ISBN 978-1-59745-398-1
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $209.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-58829-971-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $209.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-61737-860-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Functional Proteomics
Book Subtitle
Methods and Protocols
Editors
  • Julie D. Thompson
  • Christine Schaeffer-Reiss
  • Marius Ueffing
Series Title
Methods in Molecular Biology
Series Volume
484
Copyright
2008
Publisher
Humana Press
Copyright Holder
Humana Press
Distribution Rights
Distribution rights for India: CBS Publishers, New Delhi, India
eBook ISBN
978-1-59745-398-1
DOI
10.1007/978-1-59745-398-1
Hardcover ISBN
978-1-58829-971-0
Softcover ISBN
978-1-61737-860-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVIII, 596
Number of Illustrations and Tables
111 b/w illustrations, 4 illustrations in colour
Topics