Methods in Molecular Biology

Hepatitis C

Methods and Protocols

Editors: Tang, Hengli (Ed.)

  • Provides the most up-to-date technical information on the new cell culture infection system as written by leading experts in the field
  • Presents both an overview and detailed protocols on the replicon system used by virtually all the HCV labs around the world
  • Covers a wide range of multi-discipline approaches adopted for HCV research, including NMR-based structure determination and mathematical modelling
  • Broadly applicable guides valuable for studying the replication of other positive-strand RNA viruses
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-59745-394-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-58829-970-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-61737-859-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

With the development of robust cell-culturing systems capable of Hepatitis C Virus replication and infection, the past decade has witnessed an explosion of innovative research directed at understanding HCV biology in the context of the authentic replicative cycle of the virus. By providing a compilation of cutting-edge research techniques currently in use in HCV labs worldwide, Hepatitis C: Methods and Protocols, Second Edition fully updates the previous edition to reflect this progress which promises to unlock the remaining vital information regarding this human pathogen and its interactions. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Fully updated and comprehensive, Hepatitis C: Methods and Protocols, Second Edition serves as a valuable reference guide for basic and clinical researchers using HCV as a model and striving to better understand this critical disease.

Reviews

From the reviews of the second edition: “This second edition contains much that was not available for the first edition; replicons, tissue culture infection systems, infectious pseudotype particles, etc. … The chapters are authored by acknowledged experts and are authoritatively written. … Of particular value are the notes sections which highlight practical pitfalls and how to avoid them. Given the size of the hepatitis C community … this book will be a success and should adorn the shelves of many laboratories.” (David J. Rowlands, Microbiology Today, July, 2009)

Table of contents (33 chapters)

  • Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays

    Guillou-Guillemette, Helene (et al.)

    Pages 3-14

  • Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen

    Ansaldi, Filippo (et al.)

    Pages 15-23

  • Immunohistochemical Detection of HCV Proteins in Liver Tissue

    Galy, Oivier (et al.)

    Pages 25-30

  • Nomenclature and Numbering of the Hepatitis C Virus

    Kuiken, Carla (et al.)

    Pages 33-53

  • A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates

    Rolfe, Kathryn J. (et al.)

    Pages 55-71

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-59745-394-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-58829-970-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-61737-859-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Hepatitis C
Book Subtitle
Methods and Protocols
Editors
  • Hengli Tang
Series Title
Methods in Molecular Biology
Series Volume
510
Copyright
2009
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-394-3
DOI
10.1007/978-1-59745-394-3
Hardcover ISBN
978-1-58829-970-3
Softcover ISBN
978-1-61737-859-1
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XXII, 466
Number of Illustrations and Tables
73 b/w illustrations, 1 illustrations in colour
Additional Information
Originally published as volume 19 in the series: Methods Molecular Med.
Topics