Overview
- A comprehensive guide to measuring biomolecular interactions for new as well as established investigators
- Includes overviews of methods along with details to assist first time users
- Covers the necessary theory for analyzing a wide array of different kinds of binding data
- Contains descriptions of methods for studying both intermolecular and intramolecular interactions
- Includes methods for describing interactions in vivo including the effects of solvent and crowding
Part of the book series: Methods in Molecular Biology (MIMB, volume 490)
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Table of contents (14 protocols)
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Front Matter
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Back Matter
Keywords
About this book
In the areas of biochemistry and cell biology, characterizations of stability and molecular interactions call for a quantitative approach with a level of precision that matches the fine tuning of these interactions in a living cell. Supporting and up-dating previous Methods in Molecular Biology™ volumes, Protein Structure, Stability, and Interactions approaches its subject with a focus on theory and practical applications for both established methods as well as exciting new procedures. The volume presents an overview of many techniques currently used to study protein stability and interactions, including scanning and titration calorimetry, spectroscopic methods, high field NMR, and analytical ultracentrifugation. As a volume of the highly successful Methods in Molecular Biology™ series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results.
Cutting-edge and easy to reference, Protein Structure, Stability, and Interactions is an ideal guide for all scientists interested in biomolecular interactions.
Reviews
From the reviews:
"Protein Structure, Stability, and Interactions covers a thorough list of biophysical methods as applied to proteins and their environments in vivo, in vitro, and sometimes in silico. … The book begins with a tutorial on microcalorimetry by Privalov, followed by a chapter on useful protocols for urea denaturation curves … . As a whole, this book would work as a text for an advanced course in biophysical methods or as a reference book for a biophysics laboratory." (Christopher Bystroff, Journal of the American Chemical Society, Vol. 131 (23), 2009)
Bibliographic Information
Book Title: Protein Structure, Stability, and Interactions
Editors: John W. Shriver
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-367-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-58829-954-3Published: 27 October 2008
Softcover ISBN: 978-1-61737-855-3Published: 19 November 2010
eBook ISBN: 978-1-59745-367-7Published: 29 June 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 360
Number of Illustrations: 135 b/w illustrations
Topics: Protein Science, Protein Structure, Cell Biology