Methods in Molecular Biology

G-Quadruplex DNA

Methods and Protocols

Editors: Baumann, Peter (Ed.)

  • Includes coverage of a wide range of methodologies to study G-quadruplex structures
  • Provides detailed protocols which ensure successful re-recreation of experimental procedures
  • Supplies step-by-step instructions in order to enable researchers to set up their own high throughput screens for G-quadruplex ligands
  • Explores computational approaches for the detection of G-quadruplex-forming structures
  • Examines state-of-the-art physical, chemical, and biological methods
  • Contains tricks of the trade and valuable troubleshooting advice
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-59745-363-9
  • Digitally watermarked, DRM-free
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  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-58829-950-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study. In G-Quadruplex DNA: Methods and Protocols, experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular recognition. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, G-Quadruplex DNA: Methods and Protocols promises to be a useful resource for those familiar with G-quartets as well as an easy entry point for those researchers from diverse fields who are just developing an interest in the exciting implications of G-quadruplex DNA.

Reviews

From the reviews:

“The book G-Quadruplex DNA … provides an excellent collection of important methods for the study of the DNA G-quadruplex structures. … I definitely recommend this book, which can be considered an outstanding source of information that addresses several problems … in the investigation of G-quadruplex structures. The book is appropriate for everyone working in the area of G-quadruplex research, including biologists, biochemists and chemists since it is a worthy guide for both beginners and experienced scientists interested in studying this increasingly attractive field.” (Aldo Galeone, ChemBioChem, Issue 11, September, 2010)

Table of contents (15 chapters)

  • G-Quadruplexes: From Guanine Gels to Chemotherapeutics

    Bryan, Tracy M. (et al.)

    Pages 1-16

  • Molecular Modeling and Simulation of G-Quadruplexes and Quadruplex-Ligand Complexes

    Haider, Shozeb (et al.)

    Pages 17-37

  • Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential

    Ryvkin, Paul (et al.)

    Pages 39-50

  • Preparation of G-Quartet Structures and Detection by Native Gel Electrophoresis

    Moon, Ian K. (et al.)

    Pages 51-63

  • Biochemical Techniques for the Characterization of G-Quadruplex Structures: EMSA, DMS Footprinting, and DNA Polymerase Stop Assay

    Sun, Daekyu (et al.)

    Pages 65-79

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-59745-363-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-58829-950-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
G-Quadruplex DNA
Book Subtitle
Methods and Protocols
Editors
  • Peter Baumann
Series Title
Methods in Molecular Biology
Series Volume
608
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-363-9
DOI
10.1007/978-1-59745-363-9
Hardcover ISBN
978-1-58829-950-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 276
Topics