Methods in Molecular Biology

Wnt Signaling

Volume 1: Pathway Methods and Mammalian Models

Editors: Vincan, Elizabeth (Ed.)

  • Records our progress to date in understanding the intricacies of Wnt signaling
  • Includes assays for and models of both canonical and non-canonical Wnt signaling pathways
  • Contributing authors are either current or past members of the main laboratories that have shaped the Wnt field
  • Includes detailed methods as well as overviews, providing easy to follow protocols as well as a background/current status of the field
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-59745-249-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-58829-912-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-61737-841-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Since their discovery, Wnt signaling molecules have been shown to control key events in embryogenesis, to maintain tissue homeostasis in the adult and, when aberrantly activated, to promote human degenerative diseases and cancer, thus making them a vital area of study.  Wnt Signaling: Methods and Protocols examines both biochemical assays and vertebrate and invertebrate model systems to provide a point of reference to current molecular protocols and the diverse model systems employed to study this important signaling pathway.  In Volume 1, Pathway Methods and Mammalian Models, assays to measure activation of the diverse Wnt signaling pathways are explored, along with a selection of models and strategies used to study mammalian Wnt/FZD fuction, from protein-protein interaction and simple cell line models to organoid cultures and mouse models.  As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain readily reproducible laboratory protocols, complete with lists of necessary equipment and reagents and the Notes section, which reveals helpful troubleshooting tips.

 

Comprehensive and cutting-edge, Wnt Signaling: Methods and Protocols collects the expertise and knowledge of many leaders in the field to produce this invaluable two-volume resource. 

Table of contents (18 chapters)

  • The Canonical Wnt/β-Catenin Signalling Pathway

    Nick Barker

    Pages 5-15

  • Isolation and Application of Bioactive Wnt Proteins

    Karl H. Willert

    Pages 17-29

  • Purification and Wnt-Inhibitory Activities of Secreted Frizzled-Related Proteins

    Vladimir Wolf, Yoshimi Endo, Jeffrey S. Rubin

    Pages 31-44

  • Measuring GSK3 Expression and Activity in Cells

    Adam R. Cole, Calum Sutherland

    Pages 45-65

  • Inhibition of Glycogen Synthase Kinase-3

    Andrei V. Ougolkov, Daniel D. Billadeau

    Pages 67-75

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-59745-249-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-58829-912-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-61737-841-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Wnt Signaling
Book Subtitle
Volume 1: Pathway Methods and Mammalian Models
Editors
  • Elizabeth Vincan
Series Title
Methods in Molecular Biology
Series Volume
468
Copyright
2008
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-249-6
DOI
10.1007/978-1-59745-249-6
Hardcover ISBN
978-1-58829-912-3
Softcover ISBN
978-1-61737-841-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 336
Number of Illustrations and Tables
74 b/w illustrations, 2 illustrations in colour
Topics