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Electroporation Protocols

Preclinical and Clinical Gene Medicine

  • Book
  • © 2008

Overview

Editors:
  1. Shulin Li

    1. Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, USA

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  • Synthesizes electroporation, electroporation chemotherapy, and electroporation gene therapy, with a focus on the latter
  • Contains step-by-step instructions that ensure successful results
  • Provides notes on troubleshooting and avoiding known pitfalls

Part of the book series: Methods in Molecular Biology (MIMB, volume 423)

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Table of contents (40 protocols)

  1. Front Matter

    Pages i-xxi
    Download chapter PDF

  3. In Vitro Targeted Gene Delivery via Electroporation

    1. Nucleofection of Human Embryonic Stem Cells

      • Henrike Siemen, Lars Nolden, Stefanie Terstegge, Philipp Koch, Oliver Brüstle
      Pages 131-138

    2. Delivery of Whole Tumor Lysate into Dendritic Cells for Cancer Vaccination

      • Linda N. Liu, Rama Shivakumar, Cornell Allen, Joseph C. Fratantoni
      Pages 139-153

    3. Delivery of Tumor-Antigen-Encoding mRNA into Dendritic Cells for Vaccination

      • Annelies Michiels, Sandra Tuyaerts, Aude Bonehill, Carlo Heirman, Jurgen Corthals, Kris Thielemans
      Pages 155-163

    4. Delivery of DNA into Natural Killer Cells for Immunotherapy

      • Kathrin Schoenberg, Hans-Ingo Trompeter, Markus Uhrberg
      Pages 165-172

    5. Electroporation of Adherent Cells In Situ for the Study of Signal Transduction and Gap Junctional Communication

      • Leda Raptis, Adina Vultur, Heather L. Brownell, Evangelia Tomai, Aikaterini Anagnostopoulou, Rozanne Arulanandam et al.
      Pages 173-189

    6. Delivery of DNA into Adipocytes within Adipose Tissue

      • James G. Granneman
      Pages 191-195

  4. In Vivo Targeted Gene Delivery via Electroporation

    1. Delivery of DNA into Muscle for Treating Systemic Diseases: Advantages and Challenges

      • Capucine Trollet, Daniel Scherman, Pascal Bigey
      Pages 199-214

    2. Delivery of DNA into Skeletal Muscle in Large Animals

      • Patricia A. Brown, Amir S. Khan, Ruxandra Draghia-Akli
      Pages 215-224

    3. Delivery of DNA into Skin via Electroporation

      • Babu M. Medi, Jagdish Singh
      Pages 225-232

    4. Electroporation-Mediated Gene Delivery to the Lungs

      • Rui Zhou, James E. Norton, David A. Dean
      Pages 233-247

    5. Delivery of DNA into Bladder via Electroporation

      • Masaki Yoshida, Hitoshi Iwashita, Masayuki Otani, Koichi Masunaga, Akito Inadome
      Pages 249-257

    6. Analysis of Gene Function in the Retina

      • Takahiko Matsuda, Constance L. Cepko
      Pages 259-278
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Keywords

About this book

At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields. In Electroporation Protocols: Preclinicaland Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease. Following the successful Methods in Molecular Biology™ series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology.

Editors and Affiliations

  • Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, USA

    Shulin Li

Bibliographic Information

  • Book Title: Electroporation Protocols

  • Book Subtitle: Preclinical and Clinical Gene Medicine

  • Editors: Shulin Li

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-194-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-877-5Published: 01 February 2008

  • Softcover ISBN: 978-1-61737-820-1Published: 10 December 2010

  • eBook ISBN: 978-1-59745-194-9Published: 28 March 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXII, 524

  • Number of Illustrations: 141 b/w illustrations, 1 illustrations in colour

  • Topics: Biochemistry, general, Animal Genetics and Genomics, Cell Biology, Animal Biochemistry, Biotechnology

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