Methods in Molecular Biology

RNAi

Design and Application

Editors: Barik, Sailen (Ed.)

  • Provides step-by-step instructions in a laboratory bench protocol format
  • Includes detailed lists of the necessary equipment and reagents
  • Offers tips on troubleshooting from actual experience of the experts
  • Features example data set in most chapters
  • Covers a variety of organisms and applications
see more benefits

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-59745-191-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.00
price for USA
  • ISBN 978-1-58829-874-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA
  • ISBN 978-1-61737-819-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology.  In RNAi: Design and Applications, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research.  Divided into two parts, this comprehensive volume covers fundamentals including designs of RNAi, biochemical assay protocols for the major components of RNAi, and study of potential off-target effects, followed by an extensive section covering various applications of RNAi in diverse model organisms and systems, from antiviral and anticancer applications to altering flower color in plants. 

Cutting edge and clearly written, RNAi: Design and Applications enables a researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field.

Table of contents (18 chapters)

  • Principles of Dicer Substrate (D-siRNA) Design and Function

    Amarzguioui, Mohammed (et al.)

    Pages 3-10

  • Expression, Purification, and Analysis of Recombinant Drosophila Dicer-1 and Dicer-2 Enzymes

    Ye, Xuecheng (et al.)

    Pages 11-27

  • In vitro RNA Cleavage Assay for Argonaute-Family Proteins

    Miyoshi, Keita (et al.)

    Pages 29-43

  • Identifying siRNA-Induced Off-Targets by Microarray Analysis

    Anderson, Emily (et al.)

    Pages 45-63

  • Hydrodynamic Delivery of siRNA in a Mouse Model of Sepsis

    Wesche-Soldato, Doreen E. (et al.)

    Pages 67-73

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-59745-191-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.00
price for USA
  • ISBN 978-1-58829-874-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA
  • ISBN 978-1-61737-819-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
RNAi
Book Subtitle
Design and Application
Editors
  • Sailen Barik
Series Title
Methods in Molecular Biology
Series Volume
442
Copyright
2008
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-191-8
DOI
10.1007/978-1-59745-191-8
Hardcover ISBN
978-1-58829-874-4
Softcover ISBN
978-1-61737-819-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 270
Number of Illustrations and Tables
45 b/w illustrations, 3 illustrations in colour
Topics