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Agrobacterium Protocols

Volume II

  • Book
  • © 2007

Overview

Editors:
  1. Kan Wang

    1. Center for Plant Transformation, Plant Science Institute, Iowa State Universtiy, Ames
      Department of Agronomy, Iowa State Universtiy, Ames

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 344)

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Table of contents (41 protocols)

  1. Front Matter

    Pages i-xxiii
    Download chapter PDF

  2. Root Plants

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Carrot (Daucus carota L.)

      • Owen Wally, Jayaraj Jayaraman, Zamir K. Punja
      Pages 3-12

    3. Cassava (Manihot esculenta Crantz)

      • Weston Msikita, Uzoma Ihemere, Dimuth Siritunga, Richard T. Sayre
      Pages 13-24

    4. Potato (Solanum tuberosum L.)

      • Steve Millam
      Pages 25-35

    5. Sweet Potato [Ipomoea batatas (L.) Lam.]

      • Guo-qing Song, Ken-ichi Yamaguchi
      Pages 37-44

  3. Turf Grasses

    1. Front Matter

      Pages 45-45
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    2. Bermudagrass (Cynodon spp.)

      • Yaxin Ge, Zeng-Yu Wang
      Pages 47-53

    3. Perennial Ryegrass (Lolium perenne L.)

      • Fredy Altpeter
      Pages 55-64

    4. Switchgrass (Panicum virgatum L.)

      • Mariya N. Somleva
      Pages 65-74

    5. Tall Fescue (Festuca arundinacea Schreb.)

      • Yaxin Ge, Zeng-Yu Wang
      Pages 75-81

    6. Turf Grasses

      • Chip Longo, Colin Lickwar, Qian Hu, Kimberly Nelson-Vasilchik, David Viola, Joel Hague et al.
      Pages 83-95

  4. Woody Species

    1. Front Matter

      Pages 97-97
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    2. American Elm (Ulmus americana)

      • Andrew E. Newhouse, Franziska Schrodt, Charles A. Maynard, William A. Powell
      Pages 99-112

    3. Cork Oak Trees (Quercus suber L.)

      • Rubén Álvarez, Mariano Toribio, Millán Cortizo, Ricardo-Javier Ordás Fernández
      Pages 113-123

    4. Eucalyptus

      • Zenn-Zong Chen, Cheng-Kuen Ho, In-Suk Ahn, Vincent L. Chiang
      Pages 125-134

    5. Pine (Pinus radiata)

      • Jan Grant, Tracy Dale, Pauline Cooper
      Pages 135-141

    6. Poplar (Populus spp.)

      • Richard Meilan, Caiping Ma
      Pages 143-151

    7. Rubber Tree (Hevea brasiliensis Muell. Arg)

      • Perumal Venkatachalam, Radha Jayashree, Karumamkandathil Rekha, Sreedharannair Sushmakumari, Sankaren Sobha, Parukkuttyamma Kumari Jayasree et al.
      Pages 153-164

  5. Tropic Plants

    1. Front Matter

      Pages 165-165
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Keywords

About this book

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Reviews

From the reviews of the second edition:

"This is, as the title suggests, a laboratory manual with protocols for using Agrobacterium tumefaciens to transform plant species … . the book will be a valuable addition to the laboratory bookshelf. The protocols … are clearly set out and the notes explain things … . I was also pleased that some chapters mentioned the need to consider regulatory issues regarding disposal of genetically modified materials, something that is often ignored in lab manuals." (Heather Macdonald, SGM – Society for General Microbiology, 2007)

Editors and Affiliations

  • Center for Plant Transformation, Plant Science Institute, Iowa State Universtiy, Ames

    Kan Wang

  • Department of Agronomy, Iowa State Universtiy, Ames

    Kan Wang

Bibliographic Information

  • Book Title: Agrobacterium Protocols

  • Book Subtitle: Volume II

  • Editors: Kan Wang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1597451312

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-843-0Published: 01 September 2006

  • Softcover ISBN: 978-1-61737-803-4Published: 09 December 2010

  • eBook ISBN: 978-1-59745-131-4Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: 512

  • Number of Illustrations: 81 b/w illustrations, 6 illustrations in colour

  • Additional Information: Originally published in one volume

  • Topics: Plant Sciences

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