Methods in Molecular Biology

2D PAGE: Sample Preparation and Fractionation

Volume 1

Authors: Posch, Anton

  • Contains step-by-step instructions that ensure successful results
  • Provides tricks of the trade
  • Offers notes on troubleshooting and avoiding known pitfalls
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-60327-064-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-58829-722-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
  • ISBN 978-1-61737-742-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 1 focuses on the basics of sample preparation and protein labeling techniques with an emphasis on fractionation by chemical reagents, chromatography, and electrophoresis methods. The inclusion of meticulous enrichment strategies further aids the protein researcher.

 

Invaluable for students of Biochemistry, Biomedicine, Biology and Genomics and essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delve into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field.

Table of contents (34 chapters)

  • Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization

    Goldberg, Stanley

    Pages 3-22

  • Bacteria and Yeast Cell Disruption Using Lytic Enzymes

    Salazar, Oriana

    Pages 23-34

  • Sample Solublization Buffers for Two-Dimensional Electrophoresis

    Weiss, Walter (et al.)

    Pages 35-42

  • Quantitation of Protein in Samples Prepared for 2-D Electrophoresis

    Berkelman, Tom

    Pages 43-49

  • Removal of Interfering Substances in Samples Prepared for Two-Dimensional (2-D) Electrophoresis

    Berkelman, Tom

    Pages 51-62

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-60327-064-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-58829-722-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
  • ISBN 978-1-61737-742-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
2D PAGE: Sample Preparation and Fractionation
Book Subtitle
Volume 1
Authors
Series Title
Methods in Molecular Biology
Series Volume
424
Copyright
2008
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-60327-064-9
DOI
10.1007/978-1-60327-064-9
Hardcover ISBN
978-1-58829-722-8
Softcover ISBN
978-1-61737-742-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 459
Number of Illustrations and Tables
131 b/w illustrations, 2 illustrations in colour
Topics