Methods in Molecular Biology

Glycobiology Protocols

Editors: Brockhausen, Inka (Ed.)

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About this book

Glycobiology involves studies of complex carbohydrates and posttrans- tional modifications of proteins, and has become an important interdiscip- nary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Although initial research was directed toward elucidation of the different carbohydrate structures and the enzymes synthesizing them, the field has now moved toward identifying the functions of carbohydrates. The pro- cols described in Glycobiology Protocols form a solid basis for investigations of glycan functions in health and disease. The cloning of many of the genes participating in glycosylation processes has helped to enhance our knowledge of how glycosylation is controlled, but has also added another dimension of complexity to the great heterogeneous variety of the structures of the oligos- charides of glycoproteins, proteoglycans, and glycolipids. A family of similar enzyme proteins exists for each glycosylation step. Glycosyltransferases are extremely specific for both the nucleotide sugar donor and the acceptor s- strate, but many other factors control sugar transfer, including the locali- tion and topology of enzymes, cofactors, possible chaperone proteins, and the availability of sugar acceptor substrates. The analysis of the intracellular organization of glycosylation and of the factors controlling the activities of the participating enzymes in the cell are important areas that need more research efforts. Another challenge for future research is to understand the glycodynamics of a cell, that is, how the cell responds to stimuli leading to biological and pathological changes in terms of alterations in glycosylation, and how this affects the biology of the cell.

Reviews

"...a very valuable handbook for a wide audience of specialists involved in studies of carbohydrates and carbohydrate-containing biopolymers; glycobiologists, biochemists, bioorganic chemists, and molecular and cell biologists." -Biochemistry

Table of contents (24 chapters)

  • Application of Fluorophore-Assisted Carbohydrate Electrophoresis for the Study of the Dolichol Pyrophosphate-Linked Oligosaccharides Pathway in Cell Cultures and Animal Tissues

    Gao, Ningguo

    Pages 1-12

  • Partial Purification of Mannosylphosphorylundecaprenol Synthase From Micrococcus luteus

    Rush, Jeffrey S. (et al.)

    Pages 13-30

  • Assay of Dolichyl-Phospho-Mannose Synthase Reconstituted in a Lipid Matrix

    Schutzbach, John S.

    Pages 31-41

  • O-Mannosylation in Mammalian Cells

    Endo, Tamao (et al.)

    Pages 43-56

  • Methods for Analysis of Unusual Forms of O-Glycosylation

    Nita-Lazar, Aleksandra (et al.)

    Pages 57-68

Buy this book

eBook $159.00
price for USA (gross)
valid through November 5, 2017
  • ISBN 978-1-59745-167-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $209.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-58829-553-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $209.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-61737-638-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Glycobiology Protocols
Editors
  • Inka Brockhausen
Series Title
Methods in Molecular Biology
Series Volume
347
Copyright
2007
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-167-3
DOI
10.1385/1597451673
Hardcover ISBN
978-1-58829-553-8
Softcover ISBN
978-1-61737-638-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVIII, 382
Number of Illustrations and Tables
112 b/w illustrations
Topics