Methods in Molecular Biology

Human Retrovirus Protocols

Virology and Molecular Biology

Editors: Zhu, Tuofu (Ed.)

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About this book

Advances in our understanding of human retroviruses have been occurring at a very rapid pace and have contributed significantly to the development of new biotechnologies. In Human Retrovirus Protocols: Virology and Molecular Biology, recognized international researchers describe in detail the basic and state-of-the-art methods they have optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from proven methods for the isolation and detection of human retroviruses to advanced methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from peripheral blood mononuclear cells (PBMC), blood monocytes, brain tissues, cerebrospinal fluids, semen, the vagina, and lymph nodes. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinant virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection. The protocols follow the highly praised Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Human Retrovirus Protocols: Virology and Molecular Biology offers novice and experienced researchers alike proven tools essential for productive study and understanding of the human retroviruses.

Reviews

"More than 20 years after the discoveries of HTLV and HIV, there appears to be a need for a consolidated reference of many of the standard and not-so-standard protocols used to characterize the human retroviruses. This book, containing some 37 detailed protocols provided by multinational authors in two parts, does an admirable job. Part I, therefore, includes guidance on isolation of HTLV, HIV and Human Foamy Virus (HFV) from peripheral blood, lymph nodes, CNS tissue and genital secretions (semen, vaginal fluids). It also describes a variety of molecular methods (largely PCR-based) to assess viral levels, integration status and drug sensitivity, plus quantification by antigen detection. At some 313 pages, Part I forms the substantial portion of the book. Part II (168 pages) guides on assessing cell and receptor tropism, construction and utilization of molecular clones and also use of microarrays to look at host and viral gene expression. The book on the whole is well illustrated with clear photographs and diagrams to support the detailed methodology, and the references at the end of each protocol are comprehensive. "-SGM Quarterly

Table of contents (37 chapters)

  • Enhanced Culture Assay for Detection and Quantitation of Latently Infected, Resting CD4+ T-Cells Carrying Replication-Competent Virus in HIV-1-Infected Individuals

    Siliciano, Janet D. (et al.)

    Pages 3-15

  • Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals

    Schuitemaker, Hanneke (et al.)

    Pages 17-24

  • Isolation of Human Immunodeficiency Virus Type 1 From Peripheral Blood Monocytes

    Gorry, Paul R. (et al.)

    Pages 25-33

  • Isolation, Propagation, and HIV-1 Infection of Monocyte-Derived Macrophages and Recovery of Virus From Brain and Cerebrospinal Fluid

    Gorantla, Santhi (et al.)

    Pages 35-48

  • Isolation and HIV-1 Infection of Primary Human Microglia From Fetal and Adult Tissue

    Borgmann, Kathleen (et al.)

    Pages 49-70

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-59259-907-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.00
price for USA
  • ISBN 978-1-58829-495-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.00
price for USA
  • ISBN 978-1-61737-599-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Human Retrovirus Protocols
Book Subtitle
Virology and Molecular Biology
Editors
  • Tuofu Zhu
Series Title
Methods in Molecular Biology
Series Volume
304
Copyright
2005
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-907-3
DOI
10.1385/1592599079
Hardcover ISBN
978-1-58829-495-1
Softcover ISBN
978-1-61737-599-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 494
Topics