Methods in Molecular Biology

Immunocytochemical Methods and Protocols

Editors: Oliver, Constance, Jamur, Maria Célia (Eds.)

  • Fully updated from previous editions to offer cutting-edge methods in immunocytochemistry
  • Includes methods applicable to the basic research laboratory as well as the clinical laboratory
  • Provides the theoretical basis to the methods
  • Contains methods that have multidisciplinary applications
  • Suitable for both novice and experienced investigators
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-59745-324-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-58829-463-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-61779-682-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The landscape of immunocytochemistry has significantly evolved in past years. Once a laborious technique with limited application, the introduction of new detection systems and instrumentation has facilitated the widespread use of immunocytochemical methods. In the post-genomic era, the use of immunocytochemical methods has grown to include the identification of a broad spectrum of antigens including proteins, carbohydrates and lipids from virtually every organism. In Immunocytochemical Methods and Protocols, Third Edition, expert researchers explore the modern methods employed in the field today,  now widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions, and to demonstrate the localization of subcellular components. Chapters provide methods that are designed to be general in their application and to appeal to investigators across all disciplines, including those in research and clinical settings. Composed in the highly successful Methods in Molecular Biology™ series format, chapters contain a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

 Comprehensive and ground-breaking,  Immunocytochemical Methods and Protocols, Third Edition, brings together a variety of methods that are normally limited to applications in a specific area, providing a unique and vital guide for researchers and clinicians alike.

Reviews

From the reviews of the third edition:

“This book targets not only immunologists but a very broad readership. … very well-written and will be equally useful for a complete beginner to the technique, as well as for a scientist who has been using the methods for years. … I would recommend this book in particular to researchers who start to use immunocyto/histochemistry in their work … . The book should also be considered by laboratories that would like to have a reference for reliable immunostaining protocols.” (Anna Vossenkaemper, Immunology News, August, 2010)

Table of contents (43 chapters)

  • Overview of Antibodies for Immunochemistry

    Oliver, Constance (et al.)

    Pages 3-9

  • Introduction to the Purification of Antibodies

    Grodzki, Ana Cristina (et al.)

    Pages 11-13

  • Antibody Purification: Ammonium Sulfate Fractionation or Gel Filtration

    Grodzki, Ana Cristina (et al.)

    Pages 15-26

  • Antibody Purification: Ion-Exchange Chromatography

    Grodzki, Ana Cristina (et al.)

    Pages 27-32

  • Antibody Purification: Affinity Chromatography – Protein A and Protein G Sepharose

    Grodzki, Ana Cristina (et al.)

    Pages 33-41

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-59745-324-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-58829-463-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
  • ISBN 978-1-61779-682-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Immunocytochemical Methods and Protocols
Editors
  • Constance Oliver
  • Maria Célia Jamur
Series Title
Methods in Molecular Biology
Series Volume
588
Copyright
2010
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-324-0
DOI
10.1007/978-1-59745-324-0
Hardcover ISBN
978-1-58829-463-0
Softcover ISBN
978-1-61779-682-1
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XII, 416
Number of Illustrations and Tables
40 b/w illustrations, 4 illustrations in colour
Topics