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Methods in Molecular Biology

Therapeutic Proteins

Methods and Protocols

Editors: Smales, C. Mark, James, David C. (Eds.)

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eBook $189.00
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  • ISBN 978-1-59259-922-6
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Hardcover $239.00
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  • ISBN 978-1-58829-390-9
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Softcover $239.00
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About this book

With the completion of the human genome, the number of protein-based drug candidates has increased dramatically, along with the need to find methods for producing these proteins on a scale large enough to meet demand without compromising the authenticity of the final product. In Therapeutic Proteins: Methods and Protocols, world-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, Therapeutic Proteins: Methods and Protocols offers both novice and experienced researchers the latest innovative, readily reproducible techniques, as well as helpful commentaries on the development and production of therapeutic proteins, ranging from their expression to actual formulation.

Reviews

"...scientists in the area will find the material of value." - Doody's Health Sciences Book Review Journal

Table of contents (35 chapters)

  • Biopharmaceutical Proteins

    Birch, John R. (et al.)

    Pages 1-16

  • Expression of Antibody Fragments by Periplasmic Secretion in Escherichia coli

    Popplewell, Andrew G. (et al.)

    Pages 17-30

  • Secretory Production of Therapeutic Proteins in Escherichia coli

    Lee, Sang Yup (et al.)

    Pages 31-41

  • Expression of Recombinant LTB Protein in Marine vibrio VSP60

    Wang, Yili (et al.)

    Pages 43-49

  • Heterologous Gene Expression in Yeast

    Byrne, Lee J. (et al.)

    Pages 51-64

Buy this book

eBook $189.00
price for USA (gross)
  • ISBN 978-1-59259-922-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $239.00
price for USA
  • ISBN 978-1-58829-390-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $239.00
price for USA
  • ISBN 978-1-62703-848-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Therapeutic Proteins
Book Subtitle
Methods and Protocols
Editors
  • C. Mark Smales
  • David C. James
Series Title
Methods in Molecular Biology
Series Volume
308
Copyright
2005
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-922-6
DOI
10.1385/1592599222
Hardcover ISBN
978-1-58829-390-9
Softcover ISBN
978-1-62703-848-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 482
Topics