Methods in Molecular Biology

Fluorescent Energy Transfer Nucleic Acid Probes

Designs and Protocols

Editors: Didenko, Vladimir V. (Ed.)

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About this book

Although fluorescent probes and assays, which use energy transfer (ET) for monitoring DNA reactions, have multiplied in recent years, until now there have been no manuals summarizing the many different protocols and probe designs. Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is the first publication to provide such a collection. In the volume, hands-on experts-often the original developers of the technique-comprehensively describe a variety of fluorescent probes and devices that use ET, including molecular beacons, molecular break lights, TaqMan® probes, LUX™ primers, Invader® assay, aptazymes, DNAzymes, molecular machines, biosensors, and logic gates for molecular-scale computation. Merging work on nanotechnology and fluorescent probes, the authors assemble the first comprehensive treatment of all the key DNA- and RNA-based ET probes and present strategies for their optimized custom design. They discuss both fluorescence resonance energy transfer (FRET)-based and non-FRET-based constructs, and provide a complete set of techniques to monitor DNA and RNA reactions, such as hybridization, amplification, cleavage, folding, and associations with proteins, other molecules, and metal ions. Detailed protocols are provided for distance determination in protein-DNA complexes and the detection of topological DNA alterations, mutations, and single-nucleotide polymorphisms. The protocols follow the successful Methods in Molecular Biology™ series format, each offering an outline of the principles behind the technique, step-by-step detailed instructions, and comprehensive troubleshooting.
Exhaustive and state-of-the-art, Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is an invaluable resource for both novice and experienced researchers who wish to use the latest developments in fluorescent probes in any field of molecular biology.

Reviews

From the reviews:

"The content of the book provides a complete source of information in the DNA/RNA area and related fields. … In brief, the book is a useful, practical source of information. … The group of scientists and researchers … that forms the potential market for this book can be extended with an important category: undergraduate and graduate students and postdoctoral researchers. Some materials can be adapted for interdisciplinary courses in molecular life sciences." (Dr. Ton Visser, Molecular Biotechnology, Vol. 34, September, 2006)

"Fluorescence Energy Transfer Nucleic Acid Probes, Design and Protocols is a timely book. It is the first concise collection of fluorescence methods and assays used in exploring biomolecular structure, dynamic and interaction. … a rich compendium of information for the beginner in the field, as well as a source of ideas and inspiration for the advanced researcher. It is a book that should be on the shelf in all laboratories using fluorescent-probe techniques in biochemistry and molecular biology." (Sabine Müller, ChemBioChem, Vol. 8, 2007)


Table of contents (22 chapters)

  • Selection of Fluorophore and Quencher Pairs for Fluorescent Nucleic Acid Hybridization Probes

    Marras, Salvatore A. E.

    Pages 3-16

  • Choosing Reporter-Quencher Pairs for Efficient Quenching Through Formation of Intramolecular Dimers

    Johansson, Mary Katherine

    Pages 17-29

  • Detection of DNA Hybridization Using Induced Fluorescence Resonance Energy Transfer

    Mathias Howell, W.

    Pages 33-41

  • Detecting RNA/DNA Hybridization Using Double-Labeled Donor Probes With Enhanced Fluorescence Resonance Energy Transfer Signals

    Okamura, Yukio (et al.)

    Pages 43-56

  • Oscillating Probe for Dual Detection of 5'PO4 and 5'OH DNA Breaks in Tissue Sections

    Didenko, Vladimir V.

    Pages 59-69

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-59745-069-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-58829-380-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
  • ISBN 978-1-61737-531-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Fluorescent Energy Transfer Nucleic Acid Probes
Book Subtitle
Designs and Protocols
Editors
  • Vladimir V. Didenko
Series Title
Methods in Molecular Biology
Series Volume
335
Copyright
2006
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59745-069-0
DOI
10.1385/1597450693
Hardcover ISBN
978-1-58829-380-0
Softcover ISBN
978-1-61737-531-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 372
Number of Illustrations and Tables
101 b/w illustrations, 3 illustrations in colour
Topics